基于玻璃基底的细胞培养芯片研究  

Study on Glass Cell Culture Chips

作　　者：杨才表[1] 陈强[1] 邵建波[1] 吴蕾[1] 赵建龙[2] 

机构地区：[1]中国科学院上海微系统与信息技术研究所,上海200050 [2]中国科学院研究生院,北京100039

出　　处：《生物技术通讯》2008年第1期76-79,共4页Letters in Biotechnology

基　　金：国家重点基础研究发展规划项目(2005CB724305);上海市科委纳米专项项目(0652nm016;06JC14081)

摘　　要：目的:为了对细胞进行长期观察和培养,研究细胞培养芯片的制作以及其与温度控制装置、进样系统、信号检测系统等的整合。方法:以商品化的氧化铟锡(ITO)透明导电玻璃为芯片加工的基质材料,利用光刻胶AZ4620作为玻璃湿法刻蚀的掩模层,并应用玻璃湿法刻蚀过程中的钻蚀效应,快速、低成本地加工细胞培养芯片;将此芯片与聚二甲基硅氧烷(PDMS)薄膜经氧等离子体作用后共价结合,整合温度控制系统、进样系统、信号检测系统等。结果:获得了可用于细胞培养的微系统,实现了对猪髂骨动脉内皮细胞(PIEC)在芯片内至少3 d的观察和培养。结论:制备的芯片能够用于细胞的长期观察和培养,为研究细胞迁移特性等提供了有效工具。Objective： In order to observe and culture cell in vitro in a long period, the fabrication procedures about cell culture chips on glass and its integration with temperature control system, perfusion system and signal detection system were studied. Methods： Commercially-available indium-tin-oxide（ITO） glass and a layer of AZ4620 positive photoresist were used to be substrate materials and etch mask separately. Underetch phenomenon in glass wet etch also played an important role to etch microchannels. The glass substrate was then covalenfly bonded with PDMS film after oxygen plasma process to form the cell culture chips. Besides the cell culture chips, it was necessary to integrate temperature control system, perfusion system and signal detection system in the cell culture microsystem. Results： Porcine iliac artery endothelial cell（PIEC） were cultured successfully within the cell culture system more than three days. Conclusion： The results showed that the cell culture chip could be used to observe and culture cells in vitro in a long period, which supplied a good tool for investigating the cell migration in vitro.

关 键 词：氧化铟锡玻璃 湿法刻蚀 钻蚀效应 细胞培养 

分 类 号：TN364[电子电信—物理电子学] Q813.1[生物学—生物工程]