诺帝对HPV 16亚基因永生化人宫颈上皮细胞增殖、凋亡和分化的影响  

Effects of Nordy on proliferation, apoptosis, and differentiation of HPV 16 subgenes-immortalized human endocervical cells

作　　者：陈玮[1] 赵涌[1] 赵敬[1] 卞修武[2] 

机构地区：[1]重庆医科大学病理学教研室,重庆400016 [2]第三军医大学附属西南医院病理研究所,重庆400038

出　　处：《肿瘤》2008年第1期13-16,共4页Tumor

基　　金：国家八六三计划指导项目(编号:2002AA001010)

摘　　要：目的：研究诺帝（Nordy）对HPV 16型亚基因（E6、E7）永生化人宫颈上皮细胞（H8细胞）增殖、分化和凋亡的影响。方法：MTT法检测诺帝对H8细胞增殖的抑制作用，SP法检测增殖细胞核抗原mcm 5的表达，FCM分析细胞周期和凋亡，光学显微镜、电子显微镜观察细胞形态变化，端粒重复序列扩增-酶联免疫吸附法（telomerase repeat amplification protocol-enzyme linked immunosorbent assay，TRAP—ELISA）法检测端粒酶活性变化。结果：10～100μmol/L浓度的诺帝能够不同程度地抑制H8细胞的增殖，细胞核内mcm 5的表达明显下降，可阻滞细胞周期于G0／G1期、S期细胞减少，细胞凋亡率增多，细胞形态上出现向成熟分化的趋势，端粒酶活性明显降低。结论：诺帝具有抑制H8细胞增殖，促进凋亡，诱导其分化的作用，这种作用可能是通过阻滞细胞周期，降低端粒酶活性来实现的。Objective： To investigate the effects of Nordy on proliferation, differentiation, and apoptosis of HPV 16 subgenes （E6 ,E7 ）-immortalized human endocervical cells （H8 cells ）. Methods： The inhibitory effects of Nordy on proliferation of H8 cells were measured by MTT assay. The expression of nuclear antigen mcm 5 in H8 cells was detected by immunocytochemical SP method. The effect of Nordy on cell cycle and apoptosis of H8 cells was analyzed by flow cytometry （ FCM ）. Morphological changes of H8 cells were observed by light and electron microscopy. The activity of telomerase was tested by telomeric repeat amplification protocal-enzyme linked immunosorbent assay （TRAP-ELISA）. Results：Nordy 10-100 μmol/L inhibited the proliferation of H8 cells to different extent, decreased the intracellular expression of mcm 5 protein, and arrested H8 cells in G0/G1 phase, reduced the proportion of H8 cells in S phase, and increased the apoptotic rate. Morphological examination showed that Nordy-treated H8 cells tended to differentiate into mature cells. The activity of telomerase decreased significantly after Nordy treatment. Conclusion： Nordy inhibites proliferation, activates apoptosis, and induces differentiation of H8 cells by blocking cell cycle and decreasing activity of telomerase.

关 键 词：宫颈肿瘤 去甲二氢愈创木酸 细胞凋亡 H8细胞 

分 类 号：R737.33[医药卫生—肿瘤]