RGD序列多肽与不同肿瘤细胞结合特性研究  

Comparison of combination specification of the different tumor cell with RGD peptide

作　　者：贾莹莹[1] 钱海利[2] 唐萌[1] 赵周社[3] 李宏利[3] 辛军[4] 张立生[5] 林晨[2] 

机构地区：[1]中国医学科学院协和医科大学肿瘤医院核医学科,北京100021 [2]中国医学科学院协和医科大学肿瘤研究所分子肿瘤学国家重点实验室,北京100021 [3]GE医疗系统集团(中国),北京100176 [4]中国医科大学附属盛京医院放射科,辽宁沈阳110004 [5]中国医学科学院协和医科大学肿瘤研究所临床前药理检测中心,北京100021

出　　处：《中国临床医学影像杂志》2008年第1期35-37,共3页Journal of China Clinic Medical Imaging

摘　　要：目的:通过体外细胞和动物体内研究期望获得不同肿瘤细胞整合素αvβ3受体表达的资料,以指导临床研究。材料和方法:采用荧光显微镜检测FITC标记HYNIC-RGD对人胰腺癌、肝癌、肠癌和子宫颈癌细胞的结合;用99mTc-HYNIC-RGD在小鼠乳腺和肝癌动物模型上比较了肿瘤对99mTc-HYNIC-RGD的摄取。结果:体外研究表明胰腺癌和肝癌细胞对RGD结合明显高于肠癌和子宫颈癌。注射后3小时小鼠皮下接种的乳腺癌和肝癌肿瘤组织对99mTc-HYNIC-RGD的摄取达到高峰,乳腺癌对99mTc-HYNIC-RGD摄取高于肝癌40%以上。结论:体外研究表明胰腺癌和肝癌细胞均对RGD有高的摄取,活体小鼠动物模型研究表明乳腺癌对RGD具有高的摄取。Objective： To get the αvβ3 receptor combination information of the different tumor cells with RGD peptide by vivo and vitro method, so as to guide clinical molecular imaging research. Materials and Methods： Comparison combination specification of the pancreas tumor cell, hepatic tumor cell, intestine tumor cell and cervix tumor cell with FITC-HYNIC- RGD, and acquisition data for the xenograft mice model of breast tumor and hepatic tumor cell after IV injection of ^99mTc- HYNIC-RGD. Results： Combination number of the pancreas tumor cell, hepatic tumor cell is higher than intestine tumor cell and cervix tumor cell with FITC-HYNIC-RGD. The uptake of breast tumor cell with ^99mTc-HYNIC-RGD is 40% higher than that of hepatic tumor cell after injection. Conclusion： Combination of the pancreas tumor cell, hepatic tumor cell with FITC- HYNIC-RGD is the highest. Uptake of breast tumor with xenograft mice model is higher than hepatic tumor after injection of ^99mTc-HYNIC-RGD.

关 键 词：肿瘤 肽类 同位素标记 

分 类 号：R730.44[医药卫生—肿瘤] R817.4[医药卫生—临床医学]