基因活化聚乳酸表面工程的初步研究  

Preliminary Studies on the Gene-activated Poly(D,L-lactic Acid)(PDLLA) Surface

作　　者：陈欣[1] 胡燕[1] 蔡开勇[1] 王远亮[1] 

机构地区：[1]重庆大学生物工程学院,重庆400030

出　　处：《重庆大学学报（自然科学版）》2008年第1期93-96,共4页Journal of Chongqing University

基　　金：国家自然科学基金(50603032);重庆市自然科学基金(2006BB4001)

摘　　要：运用层层自装(layer-by-layer self-assembly)技术,在聚乳酸表面形成半乳糖苷化的壳聚糖(galactosylated chitosan,GC)/DNA多层膜结构,构建基因活化的生物材料。用接触角测试仪及紫外吸收光谱仪监控多层膜的组装过程及DNA的释放行为,倒置荧光显微镜观察HepG2细胞在GC/DNA多层膜修饰的聚乳酸表面的细胞形态及生长状态。结果表明:GC/DNA的交替吸收在聚乳酸表面形成了多层膜结构,DNA在PBS缓冲液(pH=7.4,37℃)中的释放可持续42 h以上。黏附在多层膜修饰的聚乳酸表面的HepG2呈现纺锤样的细胞形态。另外,半乳糖是肝细胞表面无唾液酸糖蛋白的受体,从多层膜上释放出来的GC/DNA颗粒可被肝细胞特异识别并结合。In order to construct gene-activated biomaterial, the layer-by-layer self-assembly （LBL） technique was employed to build up GC/DNA multilayered films on the poly （D, L-lactic acid） （PDLLA） film. The assembly process and DNA release behaviors were monitored by contact angle measurement and a UV-visible spectrometer, respectively. The morphology of HepG2 cultured on the LBL-modified PDLLA surface was observed according to fluorescence microscopy. These results indicated that the alternative adsorption of GC and DNA led to a multilayered structure on the PDLLA film and DNA release in the PBS buffer （pH = 7.4） at 37℃ lasting over 42 hours. HepG2 cultured on the LBL-modified PDLLA film displayed spindle-shaped feature morphology, lactose is the ligand of an asialoglycoprotein receptor （ASPR） on the hepatocyte membrane. The GC/DNA complex released from the multilayered film can be specially recognized and binds to the hepatocyte.

关 键 词：基因活化生物材料 层层自组装技术:半乳糖苷化壳聚糖 靶向基因治疗 

分 类 号：R318[医药卫生—生物医学工程]