流式细胞术检测食管癌细胞DNA含量的临床意义  

Clinical significance of using flow cytometry(FCM) to detect the DNA content in esophageal carcinoma cells

作　　者：凌雨田[1] 许沈华[1] 朱赤红[1] 

机构地区：[1]浙江省肿瘤研究所,310022

出　　处：《浙江临床医学》2008年第1期27-28,共2页Zhejiang Clinical Medical Journal

摘　　要：目的了解食管癌细胞DNA指数(DI)、S期细胞百分率(SPF)、细胞增殖指数(PI)的临床意义。方法应用流式细胞仪检测64例食管癌手术标本的癌细胞和距癌远端食管切缘粘膜细胞DI、SPF、PI值,并结合随访资料进行临床病理分析。结果根据DI值,研究对象分为二倍体组(0.90～1.10),亚二倍体组(1.11～1.20)和异倍体组(>1.20),分别占53.1%(34/64),21.9%(14/64),25.0%(16/34)。异倍体癌细胞DI、SPF、PI值高于二倍体组(P<0.01)。食管癌异倍体组I～II期病例占18.8%(3/16),低于二倍体组52.9%(18/34)(P<0.05)。食管癌异倍体组无淋巴结转移病例占25.0%(4/16),低于二倍体组55.9%(19/34)(P<0.05)。异倍体组与亚二倍体组同时SPF>10%的患者中无淋巴结转移例数占29.4%(5/17),低于二倍体组无淋巴结转移例数52.9%(18/34),有统计学差异(P<0.05)。结论食管癌细胞DI值与临床分期、淋巴结转移密切相关。与食管癌患者生存无显著相关,同时癌细胞的SPF值与淋巴结转移密切相关。Objective 34 investigate the clinical significance of DNA index（DI）, S - phase fraction（SPF）, cell proliferation index（PI） in esophageal carcinoma cells. Methods DNA content analysis by FCM was performed in cancer cells and nlucosal cells in distal surgical margin isolated from 64 operative tissue samples to determine the DI, SPF, PI values in esophageal carcinoma cells , informations on followup were combined to make a clinical pathological ananlysis. Results Reserach subjects were divided into three groups according to DI value： diploid group （ DI ：0.90 - 1.10） , hypodilpoid or near diploid group （DI： 1.11 - 1.20）, heteroploid group （DI 〉 1.20）, accounting for 53.1% （34/64）, 21.9% （14/64）, 25.0% （16/34） separately. Di , SPF, PI value of heteroploid cancer cells were significantly higher than those of diploid cancer cells, P 〈 0.01. Heteroploid esophageal carcinoma patients with stage Ⅰ-Ⅱ were in 18.8 % （3/16）, significantly lower than thai in diploid patients, 52.9% （18/34）, P 〈 0.05. Heteroploid esophageal careinoma patients without lymph node metastasis were 25.0% （4/16）, significantly lower than that in diploid patients, 55.9% （19/34）, P 〈 0.05. Heteroploid esophageal carcinoma patients without lymph node metastasis , harboring more than 10% SPF were 29.4% （5/17）, siginificantly lower than that in diploid potients, 55.9% （ 19/34）, P 〈 0.05. However, no signifieant difference was found between non - diploid esophageal carcinoma patients without lymph node metastasis , with lower thant 10% SPF and diploid patients. No significant differences were found camparing hypodiploid or near diploid cancer cells with diploid or heteroploid cancer cells . No significant relationship of DNA content with survial of esophageal carcainoma patients was found. No Correalation between PI value and clinical pathological factors was found. Conclusions DNA content of esophageal carcinoma cells is colsely related to staging , lymph node metastasis and i

关 键 词：食管癌 DNA指数 S期细胞百分率 存活率 FCM 

分 类 号：R735.1[医药卫生—肿瘤] R64[医药卫生—临床医学]