特布他林对海水淹溺型肺水肿兔肺组织炎症反应的抑制作用  

Inhibitory action of terbutaline on inflanunatory reaction of lung tissue in rabbits with lung edema induced by seawater drowning

作　　者：彭朝胜[1] 段蕴铀[2] 丁新民[2] 孟激光[2] 靳丽[1] 

机构地区：[1]海军总医院干三科,北京100037 [2]海军总医院呼吸科,北京100037

出　　处：《中华航海医学与高气压医学杂志》2007年第6期334-338,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨特布他林对海水淹溺型肺水肿兔肺组织炎症反应的影响。方法36只机械通气的麻醉新西兰兔随机分成正常组（N组）、对照组（C组）和特布他林治疗组（T组）。C组和T组经颈动脉注入4ml／kg的配方海水，20min后C组经颈动脉注入2ml生理盐水，而T、组则注入0．15mg／kg特布他林，N组除未注入配方海水外，其余处理同C组。观察肺泡灌洗液（BALF）中TNF-α计量和中性粒细胞计数，取部分右下肺常规病理学检查，并分别用RT-PCR和ELISA检测肺组织中TNF-α、IL-1β和IL-8的mRNA表达及蛋白含量。结果病理学观察显示C组的肺组织内有大量的炎性细胞浸润，肺泡隔断裂、肺泡破裂、相互融合、肺泡大量萎陷，肺泡内有出血及透明膜形成。T组的上述改变轻于C组。T组肺组织内TNF-α、IL-1β和IL-8的mRNA表达及蛋白含量均显著低于C组（P〈0．05），BALF中TNF-α和中性粒细胞计数减少。结论特布他林可以抑制海水淹溺型肺水肿兔肺组织的TNF-α、IL-1β和IL-8，从而减轻肺组织炎症反应。Objective To investigate the effect of terbutaline on inflammatory reaction of lung tissue of rabbits with lung edema induced by seawater drowning. Methods Thirty-six New Zealand rabbits treated with mechanical ventilation and anaesthesia were randomly and equally divided into normal group （group N）, control group （group C） and terbutalin group （group T）. Except rabbits in group N,others were injected compounding seawater （4 ml/kg body weight） into lungs through carotid artery. Then twenty minutes later, the rabbits in group C were injected 2 ml normal sodium into lung through carotid artery, as which the rabbits in group N were the same. While in group T,terbutalin （0.15 mg/kg body weight） instead. The counts of neutrophil and TNF-α and neutrophile granulocyte in bronchoalveolar lavage fluid were observed. A portion of low right lung was examed by conventional pathematology. Expressions of mRNA of TNF-α、IL-1β and IL-8 in lung tissue were measured by reverse transcriptase-polymerase chain reaction （ RT-PCR）, and concentrations of their protein were detected by ELISA. Results Light microscopic findings demonstrated a marked lung injury in of group C, represented by hemorrhage, edema, thickened alveolar septum, formation of hyaline membranes and the infiltration of inflammatory cells in alveolar spaces. In group T, these changes were less pronounced. The expressions of TNF-α、IL-1β and IL-8 mRNA and the contents of their protein were significantly lower in group T than in group C （ P 〈 0.05 ）. Conclusions Terbutaline can inhibit the expressions of TNF-α、IL-1β and IL-8, and relief the inflammatory reaction Of lung tissue in rabbits with lung edema induced by seawater drowning.

关 键 词：淹溺 海水 炎症反应 特布他林 

分 类 号：R[医药卫生]