皮肤基底细胞癌和鳞状细胞癌皮损中Smad 7、Smurf 1和Smurf 2的表达  

Expression of Smad 7,Smurf 1 and Smurf 2 in basal cen carcinoma and squamous cell carcinoma

作　　者：李颖[1] 何威[1] 何云志[1] 黄海[1] 林自华[1] 吴军[1] 

机构地区：[1]第三军医大学新桥医院皮肤科,重庆400037

出　　处：《中华皮肤科杂志》2007年第12期736-739,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30371298)

摘　　要：目的探讨Smad 7、Smurf 1和Smurf 2在基底细胞癌和鳞状细胞癌皮损中表达水平的变化及其意义。方法采用实时定量聚合酶链反应和免疫组化技术分别检测基底细胞癌、鳞状细胞癌及正常人对照皮肤中Smad 7、Smurf 1和Smurf 2的表达。结果Smad 7、Smurf 1和Smurf 2在基底细胞癌和鳞状细胞癌中的表达较正常表皮显著升高。结论基底细胞癌和鳞状细胞癌皮损中Smad 7、Smurf 1和Smurf 2的表达增强可能干扰转化生长因子β(TGFβ)信号转导通路的多个环节,使TGFβ抑制上皮增生的作用不能有效发挥,从而有助于上述表皮肿瘤的异常增殖。Objective To investigate the expression and significance of Smad 7, Smurf 1 and Smurf 2 in basal cell carcinoma and squamous cell carcinoma. Methods Biopsy specimens were resected from 14 patients with basal cell carcinoma, 19 patients with squamous cell carcinoma and 30 normal controls. Quanti- tative real-time PCR and immunohistochemical techniques were utilized to assess the expression of Smad 7, Smurf 1 and Smurf 2 in these specimens. Results The gray scale for staining of Smad 7, Smurf 1 and Smurf 2 was 166.61 ± 7.11, 166.08 ± 8.71, and 166.25 ± 8.15 respectively in basal cell carcinoma, 161.66 ± 5.52, 166.84± 9.27, and 169.98± 9.48 respectively in squamous cell carcinoma. The expression levels of Smad 7, Smurf 1 and Smurf 2 were all significantly increased in basal cell carcinoma and squamous cell carcinoma in comparison with normal controls. Conclusions The over-expression of Smad 7, Smurf 1 and Smurf 2 may interfere with transforming growth factor β signaling transduction pathway through several links, therefore prevent the inhibitory effect of transforming growth factor β on epidermal proliferation, and accelerate the abnormal proliferation in above epidermal tumors.

关 键 词：癌 基底细胞 癌 鳞状细胞 SMAD 7 Smurf 1 Smurf 2 转化生长因子Β 信号转导 

分 类 号：R739.5[医药卫生—肿瘤] R739.65[医药卫生—临床医学]