铜对牙鲆CAT、SOD和GSH-PX活性的影响  

Effect of Cu^(2+) on CAT,SOD,GSH-PX of Paralichthys olivaceus

作　　者：王凡[1] 赵元凤[2] 吕景才[2] 刘长发[2] 

机构地区：[1]洛阳师范学院生命科学系,洛阳471022 [2]农业部海洋水产增养殖学与生物技术重点开放实验室

出　　处：《华中农业大学学报》2007年第6期836-838,共3页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30271029)资助

摘　　要：以鳃、肌肉和内脏组织超氧化物歧化酶SOD、过氧化氢酶CAT、谷胱甘肽过氧化物酶GSH-PX活性为指标研究了Cu2+污染对牙鲆的损伤作用。通过室内暴露试验,将牙鲆置于不同浓度(0.00、0.01、0.05、0.30、0.50、0.80 mg/L)的Cu2+溶液中10 d,测定其鳃、肌肉和内脏中过氧化氢酶CAT、谷胱甘肽过氧化物酶GSH-PX、超氧化物歧化酶SOD的活性,结果表明:随着Cu2+浓度的增高,牙鲆的3种组织中CAT、SOD、GSH-PX的活性均表现为:Cu2+浓度在0.01~0.05 mg/L出现不同程度的诱导,酶活性均高于对照组水平,Cu2+浓度大于0.05 mg/L时,各酶活性低于对照组水平。可以证实,SOD、CAT、GSH-PX活性变化可以反映牙鲆的伤害程度。The aim of this work was to study the effect of long term exposure to Cu^2＋ （0.00,0. 01, 0.05,0. 30,0. 50,0. 80 mg/L） on Paralichthys olivaceus antioxidant system in muscle, gill, viscus, by using the activities of CAT, SOD and GSH-PX as indexes. After 10 day＇s exposure experiment, the resuits showed that within the range of Cu^2＋ dosages the activities of CAT, SOD and GSH-PX of the muscle,gill,viscus in Paralichthysolivaceus were inducted in low level of Cu^2＋ 0. 01-0.05 mg/L and inhabited with the high level of Cu^2＋ （〉0. 05 mg/L）. These results also suggest the changes of the activities of SOD,CAT and GSH-PX can indicate the injured degree of Paralichthys olivaceus by Cu^2＋.

关 键 词：CU^2+ 牙鲆 CAT SOD GSH-PX 

分 类 号：X171.5[环境科学与工程—环境科学]