硒化紫球藻胞外多糖体外诱导BEL-7402肿瘤细胞凋亡的实验研究  

In vitro experimental study of apoptosis of BEL-7402 cells induced by selenium exo-polysaccharide of Porphyridium sp. (Se-PSP)

作　　者：朱劲华[1] 王敏[2] 张威[1] 李朝军[2] 张成武[2] 

机构地区：[1]江苏职工医科大学,江苏南京210029 [2]南京师范大学江苏省分子医学生物技术重点实验室,江苏南京210097

出　　处：《中国海洋药物》2007年第6期36-39,共4页Chinese Journal of Marine Drugs

摘　　要：目的研究硒化紫球藻胞外多糖对BEL-7402等肿瘤细胞系的生长及凋亡的影响。方法采用在培养液中添加亚硒酸,制备硒化紫球藻(Porphyridiumsp.)胞外多糖(Se-PSP);在体外培养条件下,用不同浓度的紫球藻胞外多糖(PSP)及硒多糖(Se-PSP)处理肿瘤细胞,并用MTT,DNA ladder,Focus等方法测定。结果与结论Se-PSP(80mg.L-1)对BEL-7402肿瘤细胞系有极为显著的抑制作用,且能诱导BEL-7402细胞凋亡。Objective To observe the effects of selenium extracellular polysaccharide of Porphyridiurn sp （Se-PSP） on the growth and apoptosis of BEL-7402 tumor cell line. Methods The Se-PSP was obtained from a sort of culture medium with some selenous acid solution. In vitro, human tumor cells were cultured in sorts of PSP or Se-PSP liquors with different concentrations, and the result of which was determined by MTT, Focus apoptosis assay and DNA fragmentation. Results and Conclusion Se-PSP（80mg · L^-1） displayed strong growth inhibitory effects against BEL-7402 human cancer cell line, and induced the apoptosis of BEL-7402 cells.

关 键 词：紫球藻 胞外多糖 硒 细胞凋亡 

分 类 号：R931.711[医药卫生—生药学] R965[医药卫生—药学]