西兰花中异硫氰酸盐对HepG-2细胞中甲胎蛋白含量的影响  

Effct on the quality of AFP in HepG-2 cells influenced by isothiocyanates in Broccoli

作　　者：季宇彬[1] 李雷[1] 邹翔[1] 刘洋[1] 胡丹[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076

出　　处：《哈尔滨医药》2007年第6期2-4,共3页Harbin Medical Journal

摘　　要：目的观察异硫氰酸盐(ITCs)对人肝癌细胞生长和细胞内甲胎蛋白变化的影响。方法以HepG-2为实验对象,分对照组、不同浓度的ITCs组,采用MTT法检测ITCs作用后细胞的生长抑制率,放射免疫法(ELISA)测定细胞中甲胎蛋白(AFP)的含量。结果异硫氰酸盐能明显抑制HepG-2细胞增殖,细胞形态呈明显的良性分化,甲胎蛋白(alpha-fe-toprotein,AFP)合成及分泌量明显下降。结论西兰花中异硫氰酸盐对HepG-2细胞的增长抑制作用可能与影响甲胎蛋白变化有关。Objective To study the effect of AFP influenced by isothiocyanates （ITCs）on human hepa toma cell HepG-2 . Methods The cancer cell was cultured in vitro and treated with ITCs of different con centration for different time ,the profile ration was studied with MTT test. AFP was detected by micro plate reader. Result lsothiocyanates can inhibit growth of HepG-2 cells and induce the cells to benign di rection, the quantity of AFP decreased as the ITCS increase. Connelusion The effect of lsothiocyanates in hibiting the growth of HepG-2 cell may connect with the variation on AFP in HepG-2 cells.

关 键 词：异硫氰酸盐 肝癌细胞 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤]