NF-κB上调偏头痛大鼠脑膜ICAM-1蛋白表达  

THE EFFECTS OF NF-ΚB ON ICAM-1 PROTEIN EXPRESSIONS OF RAT DURA MATER WITH MIGRAINE

作　　者：何秋[1] 王怀良[2] 章新华[2] 陈磊[2] 

机构地区：[1]辽宁省人民医院神经内科,110016 [2]中国医科大学基础医学院临床药理教研室

出　　处：《中国疼痛医学杂志》2007年第6期345-348,共4页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金资助项目(30271510)

摘　　要：目的:探讨核转录因子-κB(nuclear factor-kappa B,NF-κB)在偏头痛脑膜炎症反应中的作用及细胞间黏附分子-1(intercellular adhesion molecules-1,ICAM-1)表达的调控机制。方法采用静脉注射(iv)硝酸甘油(glyceryl trinitrate,GTN)法建立大鼠偏头痛模型,分为对照组、模型组、溶剂对照组、吡咯烷二硫氨基甲酸(pyrrolidine dithiocarbamate,PDTC)组。各组分别包括0.9%生理盐水或GTN iv后1.5,4h两个实验小组。应用Western印迹法分别观察PDTC对GTN iv后1.5h大鼠脑膜NF-κB蛋白表达水平与GTN iv后4h ICAM-1蛋白表达水平的影响。结果:PDTC50,100,200mg.kg-1各剂量组GTN iv后1.5h大鼠脑膜NF-κB蛋白表达量较模型组分别降低30%(P<0.05)、52%(P<0.01)和65%(P<0.01),呈剂量依赖性;PDTC50,100,200mg.kg-1各剂量组GTNiv后4h大鼠脑膜ICAM-1蛋白表达量较模型组分别降低36%(P<0.05)、71%(P<0.01)和51%(P<0.01),无剂量依赖关系。结论:NF-κB参与偏头痛时脑膜ICAM-1的蛋白合成调控,在偏头痛的脑膜炎症机制中起着重要作用。Objective ： To investigate the effects of nuclear factor-kappa B （NF-KB） on the inflammatory response of rat dura mater with migraine and on the regulation mechanisms of intercellular adhesion molecules-1 （ICAM-1）. Methods： Rat migraine model was made by glyceryl trinitrate （GTN,2μg . kg^-1. min^- 1 ） infusion for 30 min. The rats were randomly assigned to four groups ： sham operated group, model group, PDTC （pyrrolidine dithiocarbamate） group and solvent control group. Each group includes two groups in 1.5h or 4h after normal saline or GTN infusion respectively. The levels of NF-KB and ICAM- 1 protein expression in rat dura maters were detected by Western blot. Results： NF-κB protein expression levels of rat dura mater in PDTC （50, 100,200mg. kg^-1 ） pretreatment group induced a dose-dependent inhibition of 30% （ P 〈 0.05 ）, 52% （P 〈 0.01 ） and 65% （ P 〈 0.01 ）, respectively 1.5h after GTN infusion. ICAM-1 protein expression levels of rat dura mater in PDTC （50, 100,200mg. kg^-1 ） pretreatment group were decreased a dose-independent inhibition 4h after GTN infusion, respectively 36% （P 〈 0. 05 ） ,71% （P 〈 O. O1 ）and 51% （P 〈 O. O1 ）. Conclusions： NF-KB should participate regulation of ICAM- 1 protein expression in migraine and might play an important role in the inflammatory response of rat dura mater with migraine.

关 键 词：硝酸甘油 核因子-ΚB 细胞间黏附分子-1 吡咯烷二硫氨基甲酸 偏头痛 

分 类 号：R747.2[医药卫生—神经病学与精神病学]