儿童急性白血病生存素和细胞周期素B1基因的表达  

Expressions and significance of survivin and cyclin B1 gene in childhood acute leukemia

作　　者：王广州[1] 祝伟[1] 张蕾[1] 

机构地区：[1]河南省漯河市中心医院儿科,漯河市462000

出　　处：《中国医学文摘（肿瘤学）》2007年第4期367-368,共2页Journal of Chinese Medical Abstracts·Oncology

摘　　要：目的:探讨生存素、细胞周期素B1的表达与儿童急性白血病(AL)的关系。方法:采用RT-PCR法对初治或复发AL患儿4 2例和完全缓解AL(AL CR)患儿13例及非恶性疾病患儿15例骨髓单个核细胞进行基因生存素、细胞周期素B1表达的检测。结果:初治和复发AL组生存素mRNA表达水平分别为0.472和0.810;细胞周期素B1 mRNA为0.609和0.739,均高于AL CR组和对照组(均为0)(P<0.01);复发AL组生存素mRNA表达水平高于初治组,差异有统计学意义(P=0.025).生存素基因的表达与白细胞总数及临床分型有关,而与性别、年龄、免疫分型无明显相关性;细胞周期素B1表达与性别、年龄、免疫分型、白细胞总数及临床分型无明显相关关系。儿童AL中生存素与细胞周期素B1的表达呈正相关。生存素mRNA表达水平高者缓解率低。结论:儿童AL中存在生存素、细胞周期素B1异常表达,两者表达存在相关性。生存素是AL复发的高危因素;AL生存素高表达者可以降低AL的化疗敏感性,生存素水平对AL有判断疗效和预后的价值。Aim： To investigate the expressions and significance of survivin and cyclin BI gene in childhood acute leukemia （AL）. Methods： The expressions of survivin and cyclin B1 gene were examined by RT-PCR in bone marrow cells from 42 children with incipient or relapsed AL, 13 with completely remissive AL （AL CR） and 15 without malignant disease as a control group. Results： In incipient and relapsed AL groups, the expression level of survivin were 0. 472 and 0. 810, cyclin B1 were 0. 606 and 0. 739, which were remarkably higher than those in control group and AL-CR group （P〈0.01）. There was a significantly difference between incipient AL group and relapsed AL group in the expression level of survivin mRNA （P=0.025）. The expression of survivin gene was associated with initial white blood cell （WBC） count and clinical types of AL. The expression of cyclin B1 gene was not correlated with age, sex, immunogenic type, initial WBC count and clinical types of AL. There was a significantly positive correlation between the expression of survivin gene and cyclin B1 in Childhood AL （r=0. 6864, P〈0. 01）. Patients with high survivin expression level achieved a lower rate of complete remission （CR） than those with low expression level of survivin. Conclusion： Survivin and cyclin B1 gene are expressed widely in specimens of AL, and there is a positive correlation between them. Overexpression of survivin may imply the lower sensitivity of the leukemic cells to chemotherapy and the higher risk of relapse. Overexpression of survivin might be an index of treatment effect, and might be related to poor prognosis.

关 键 词：生存素 细胞周期素B1 急性白血病 逆转录聚合酶链反应 

分 类 号：R739.6[医药卫生—肿瘤] R733.71[医药卫生—临床医学]