压力对髁突软骨细胞白介素4、6分泌的影响  

Effects of mechanical pressure on the expression of interleukin-4 and interleukin-6 in mandibular condylar chondrocytes

作　　者：张旻 陈永进[1] 吕昕[1] 赵蕊妮[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2007年第12期684-687,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30000035)

摘　　要：目的:探讨下颌骨髁突软骨细胞白细胞介素4和白细胞介素6的表达随机械力的变化过程。方法:体外培养下颌骨髁突软骨细胞,采用可控液压细胞加载装置90kPa压强下分别加载60、360min,免疫组化染色观察加压前后白细胞介素4和白细胞介素6的表达、分布变化。结果:正常对照的MCC中IL-4和IL-6只有很弱的表达,90kPa加压60min后IL-6在胞浆、胞核中表达强阳性,IL-4也在胞浆、胞核中阳性表达;加压时间延长至360min后IL-6表达较60min时有所减弱,而IL-4的表达比60min时持续增强。结论:适宜的压力刺激可引起髁突软骨细胞中白介素4、6表达的增强。AIM：To investigate the expression of interleukin-4（IL-4） and interleukin-6（IL-6） in chondrocytes（MCC） of rabbit mandibular condyle.under mechanical pressure METHODS：In vitro cultured MCC from two-week-old New Zealand rabbits were incubated.By celluar strain unit under controlled hydraulic pressure of 90kPa for 60 min and 360min,the expression and distribution of IL-4 and IL-6 were observed by immunocytochemical staining.RESULTS：Only small amount of IL-4 and IL-6 were detected in MCC of the control group.After been pressed under 90kPa for 60min,the expression of IL-6 was extremely strong in both cytoplasm and nuclei and that of IL-4 showed also distinct difference.When the pressure prolonged to 360 min,the expression of IL-6 decreased a little and that of IL-4 increased persistently.CONCLUSION：Feasible pressure could promote the expression of IL-4 and IL-6.

关 键 词：髁突软骨细胞 压力 白细胞介素 

分 类 号：R780.2[医药卫生—口腔医学]