阿托伐他汀对心肌梗死后大鼠转化生长因子-β_1和白介素-1β转录水平的影响  

Effects of atorvastain on transcription levels of TGF-β_1 and IL-1βin rats post myocardial infarction

作　　者：王艾丽[1] 陈玲玲 胡剑峰[1] 程新耀[1] 

机构地区：[1]武汉大学中南医院超声心动图室 [2]麻城市妇幼保健院儿科

出　　处：《中国临床药理学与治疗学》2007年第12期1381-1384,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨心肌梗死后细胞转化生长因子-β_1 (TGF-β_1)和白介素-1β(IL-1β)的转录水平以及阿托伐他汀对其的影响。方法:结扎冠脉左前降支造成急性心梗模型,随机分为心肌梗死组和阿托伐他汀治疗组,每组21只,另设13只为假手术组。阿托伐他汀治疗组于手术后2 d起灌胃给药,心肌梗死组和假手术组只给予等量清水灌胃。第1周后各组取3只大鼠检测TGF-β_1和IL-1βmRNA的转录水平变化;第6周后行超声心动图、心室重塑、TGF-β_1和IL- 1βmRNA的测定。结果:与心肌梗死组相比,阿托伐他汀治疗组左室舒张末期内径(LVEDD)及左、右心室肥厚指数降低;短轴缩短率(FS)和左室射血分数(EF)均增加(P<0.05);心肌梗死后第1周心肌梗死组和阿托伐他汀治疗组梗死区周边TGF-β_1和IL- 1βmRNA的转录较假手术组有不同程度增高(P<0.05)。梗死后第6周心肌梗死组IL-1βmRNA的转录与第1周相比有明显降低,而TGF-β_1 mRNA仍呈高转录水平(P<0.05);与假手术组相比两细胞因子仍增高(P<0.05)。梗死后第6周阿托伐他汀治疗组IL-1β和TGF-β_1 mRNA的表达较第1周相比皆降低(P<0.05);与心肌梗死组相比也降低(P<0.05)。结论:阿托伐他汀能降低急性心梗大鼠心肌TGF-β_1和IL-1β转录水平,减轻心室重塑,改善心功能。AIM： To investigate the effects of atorvastain on transcription levels of cytokines of TGF-β1 and IL-1β post acute myocardial infarction in rats. METHODS： Rats with acute myocardial infarction were randomly divided into myocardial infarction group and＇atorvastain treatment group （21 in each group）. 13 normal rats were used as non-infarction controls（ sham group）. In treatment group, atorvastain was intragastrically administratered at the 2 nd day after myocardial infarction. After 1 week, TGF-β1 and IL-1β mRNA were determined in each group. After 6 weeks, the cardiac function, related ventricular remodeling parameters and TGF-β1 and IL-1β mRNA were determined as well. RESULTS： Compared with myocardial infarction group, atorvastain significantly decreased LV end-diastolic dimension （LVEDD）. However, the ventricular remodeling parameters, ejection fraction （EF） and fractional shortening （FS） were significantly increased in atorvastain treatment group, compared with myocardial infarction group （P 〈 0.05 ）. The mRNA transcription levels of TGF-β1 and IL-1β in both myocardial infarction and atorvastain treatment groups were significantly higher than those in the sham group （ P 〈 0.05）. After 6 weeks, IL-1β and TGF-β mRNA levels were increased in myocardial infarction group. After treatment with atorvastain for 6 weeks, the mRNA levels of IL-1β and TGF-β were decreased compared with myocardial infarction group（P 〈0.05）. CONCLUSION： Atorvastain can downregulate TGF-β1 and IL-1β transcription and may be effective in preventing ventrieular remodeling after myocardial infarction in rats.

关 键 词：心肌梗死 心室重塑 转化生长因子-β1 白 介素-1Β 阿托伐他汀 

分 类 号：R575.2[医药卫生—消化系统] R589.2[医药卫生—内科学]