红果人参叶片RNA的提取  

Extraction of Total RNA from Panax Ginseng C.A Meyer.cv.Hongguo Leaves

作　　者：杨成君[1] 王军[1] 周莉[1] 刘关君[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《生物技术通报》2008年第1期136-139,共4页Biotechnology Bulletin

基　　金：国家林业局野生动植物保护项目(010-413255)

摘　　要：提取高质量的RNA是进行人参植物分子生物学研究的必要前提。利用CTAB法、Trizol法、Bizol法和SDS法,从红果人参叶片中提取了总RNA,通过紫外分光光度法和凝胶电泳检测,结果显示:四种方法得到的纯度都比较高,OD260/OD280值都在1.7～2.0之间;SDS法提取的RNA,凝胶电泳显示28S条带是18S条带亮度两倍,而且无污染,好于其他三种方法。通过RT-PCR,ds cDNA片段条带弥散分布大小在0.2～3.0kb,从而进一步验证了SDS法提取的RNA质量。Extraction of high-quality RNA is a prerequisite to study panax ginseng cv. Hongguo plant molecular biology,Basing on the methods of CTAB,Trizol,Bizol,and SDS,total RNA was isolated from panax ginseng cv. Hongguo leaves,The quality of total RNA was analyzed with agarose gel electrophoresis and UV spectrophotometer. The result indicated that the purity of RNA extracted was high by four methods,all of A260/A280 was range from 1.7 to 2.0; the sharpness of 28S bands was twice than 18S of bands by using SDS method,and was not contaminative. The other methods were less than that SDS method. After RT-PCR,ds cDNA bands size was range from 0.2kb to 3.0kb,the results further demonstrated that the purity and integrality of total RNA isolated by using SDS method were significantly satisfactory for the demands of molecular biological research.

关 键 词：人参叶片 RNA提取 RT-PCR 

分 类 号：Q943.2[生物学—植物学]