过氧化物酶体增殖物激活受体γ在大肠肿瘤中的表达及意义  

Expression and Significance of PPARγ in Large Intestine Carcinoma

作　　者：李传凤[1] 李军[1] 吕愈敏[1] 

机构地区：[1]北京大学第三医院消化科,100083

出　　处：《肿瘤防治研究》2008年第1期23-26,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30370633)

摘　　要：目的在蛋白及mRNA水平确定过氧化物酶体增殖物激活受体γ(peroxiome proliferator-activated receptor-γ,PPARγ)在大肠肿瘤中的表达。方法结肠镜下活检标本,分为正常大肠粘膜组、大肠腺瘤组及大肠癌组,免疫组化及RT-PCR方法检测各组PPARγ的表达。结果正常大肠粘膜中表达PPARγ蛋白的细胞主要是接近肠腔,分化良好的腺上皮细胞;而在大肠腺瘤中,PPARγ阳性的细胞呈弥漫分布。PPARγ蛋白及mRNA在大肠癌中的表达显著增加,明显高于大肠腺瘤和正常大肠粘膜(P<0.05)。PPARγ蛋白及mRNA在正常大肠粘膜及腺瘤中的表达无差异(P>0.05)。PPARγmRNA在重度异型增生腺瘤中的表达高于正常大肠粘膜(P<0.05),接近于在大肠癌中的表达(P>0.05)。结论大肠腺瘤最先发生PPARγ表达部位的变化,PPARγ在重度异型增生的大肠腺瘤及大肠癌中的表达水平较高,表明PPARγ可能在早期即参与大肠癌的发生发展。Objective To investigate the expression of PPARγ protein and mRNA in large intestine cancer. Methods Human normal mucosa, colorectal adenoma and colorectal cancer specimens were taken from colonoscopic examination. Histopathological immunohistochemistry staining was performed to examine the expression and localization of PPARγ protein and RT-PCR was used to detect the expression of PPARγ mRNA in human normal mucosa, colorectal adenoma and cancer. Results In human normal mucosa, PPARγ protein was expressed in epithelial cells facing the colonic lumen, which were well differentiated. However, it was expressed diffusely in adenoma. A strong expression of PPARγ protein and mRNA was found in cancer cells, which was significant higher than colorectal adenoma and normal mucosa （P〈0.05）. The expression level of PPARγ protein and mRNA in normal mucosa and colorectal adenoma had no significant difference （P〈0. 05）. The expression of PPARγ mRNA in high-grade dysplasia subgroup had no difference with colorectal cancer （P〉0. 05） and was higher than that of normal mucosa （P〈0.05）. Conehtsion The location of PPARγ changed first in colorectal adenoma. PPARγ was expressed at relatively higher levels in high-grade dysplasia and colorectal cancer. These data indicate that PPARγ may involve early in the development of colorectal cancer.

关 键 词：过氧化物酶体增殖物激活受体Γ 大肠腺瘤 大肠癌 

分 类 号：R735.3[医药卫生—肿瘤]