豇豆边缘细胞对铝胁迫的响应  

Response of root border cell in cowpea to aluminum stress

作　　者：徐根娣[1] 陈敏燕[1] 刘鹏[1] 蔡妙珍[1] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2008年第1期66-71,共6页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家科技攻关项目(2004BA52506);国家自然科学基金资助项目(30540056);浙江省自然科学基金资助项目(405185;303461)

摘　　要：以豇豆为材料,采用悬空培养法,研究了豇豆边缘细胞发育的特性及铝毒对豇豆边缘细胞的影响.研究表明,豇豆边缘细胞的发育和初生根的发育几乎同步,在豇豆根长15 mm时边缘细胞数目达到最大值,在根长1 mm时根冠的果胶甲基酯酶(PME)活性达到最大.豇豆离体边缘细胞在含铝溶液中存活不到24 h;而黏附于根尖的边缘细胞在同样浓度的铝溶液处理下,48 h后还保持85%的存活率.在处理时间相同的条件下,不同浓度的铝溶液处理间豇豆PME的活性不存在明显的差异,说明PME的活性受铝的影响较少,而主要与边缘细胞的脱落有关.The effect of aluminum （Al） toxin on production and development of root border cells were investigated with cowpea （Vigna ungniculata ssp. sesquipedalis） under hanging in the air culture. The results showed that the first border cell formation almost synchronized with root tip emergence in cowpea. The number of border cell reached the max when root length was 15 mm and when root length was 1 mm the PME activity of root cap achieved the max. Border cells parting from root cap would be all dead within 24 hours but the attached root cap still had 85% livability after 48 hours in the same aluminum concentration. The PME activity difference among different Al treatments with the same treatment time was not significant, which was related to the numbers of border cell and not evidently affected by aluminum.

关 键 词：豇豆 边缘细胞 果胶甲基酯酶 铝毒 根冠 

分 类 号：S52[农业科学—作物学] Q945[生物学—植物学]