虫草菌丝提取物对大鼠脂肪性肝炎与肝纤维化的影响  

Effects of Cordyceps mycelium extract on steatohepatitis and liver fibrosis in rats

作　　者：孙保木[1] 宣红萍[1] 李风华[2] 胡义扬[1] 刘平[1] 刘成海[1] 

机构地区：[1]上海中医药大学附属曙光医院肝病研究所,上海201203 [2]上海中医药大学科技实验中心

出　　处：《中国新药与临床杂志》2008年第1期6-11,共6页Chinese Journal of New Drugs and Clinical Remedies

基　　金：上海市科委重点基础项目(04JC14069);教育部新世纪优秀人才资助计划(NCET-04-0437);上海市教育委员会E-研究院建设计划(E03008);国家重点基础发展计划(973计划)课题(2006CB504801);上海市重点学科建设项目(Y0302)部分资助

摘　　要：目的探讨虫草菌丝提取物对大鼠脂肪性肝炎与盱纤维化的作用。方法Wistar雄性大鼠,随机分为3 wk和6 wk的正常组、模型组和虫草菌丝组。造模后3 wk和6 wk分别观察肝组织脂肪变性、炎症与胶原沉积;分析血清ALT、AST水平与TBil、Alb、TG、TC含量;肝组织TG、MDA、GSH含量与SOD、γ-GT活性、羟脯氯酸(Hyp)含量及肝组织α-SMA表达。结果3 wk、6 wk模型大鼠血清ALT、AST、γ-GT活性与TBil含量均升高(P<0.05),Alb、TG含量下降(P<0.01),肝组织Hyp、TG、MDA含量明显增加(P<0.01),α-SMA表达增加。3 wk虫草菌丝组改善模型大鼠肝组织脂肪变性与炎症;降低血清TBil含量,提高血清Alb含量;降低肝组织TG、MDA含量(P<0.01),提高肝组织GSH含量与SOD活性(P<0.05)。6 wk虫草菌丝组减轻模型大鼠肝纤维化;降低血清ALT、AST水平(P<0.05);降低肝组织TG、MDA含量和γ-GT活性(P<0.05),提高SOD活性。虫草菌丝3 wk和6 wk组均明显减少α-SMA表达(P<0.05),且6 wk作用较3 wk更为明显。结论虫草菌丝提取物显著改善大鼠脂肪性肝炎、抑制脂肪性肝纤维化,其作用机制与抗肝脂质过氧化、抑制肝星状细胞活化有关。AIM To evaluate the effects of Cordyceps mycelium extract on steatohepatitis and liver fibrosis in rats. METHODS Male Wistar rats were randomly divided into 6 groups： normal, model control and Cordyceps mycelium extract treated groups at 3 wk and 6 wk, respectively. After administration of Cordyceps mycelium extract （ 10 mL ·kg^-1· d^-l） at 3 wk and 6 wk, respectively, hepatic tissue of inflammation and fatty degeneration were stained with HE and Oil red O stain, collagen deposition with Sirius red. The liver function parameters including serum ALT, AST activity, Alb and TBil levels, and serum contents of TG and TC were determined. TG, SOD, MDA, γ-GT and GSH of liver tissues were also measured. Hepatic hydroxyproline （Hyp） content was detected with Jamall＇ method. The expression of α-SMA was analyzed by Western blotting and immunohisto-pathological staining. RESULTS Both 3 wk and 6 wk models had remarkable increase in serum ALT, AST activity and TBil level （P 〈 0.05） , decrease of Alb and TG content （P 〈 0.01） , with conspicuouse fatty degeneration and collagen deposition, together with higher Hyp, TG, MDA content and γ- GT activity, and lower GSH content and SOD activity （P 〈 0.05） . and simultaneously with increase of α- SMA expression. The 3 wk treated group showed mild hepatic inflammation and fatty degeneration, and lower total TBil levels, TG and MDA content, and higher Alb and GSH content and SOD activity than those in 3 wk model group （P 〈 0.01 ）. The 6wk treated group showed mild fibrosis, lower ALT and AST activity, TG and MDA content and γ-GT activity and higher SOD activity than those in the 6 wk model group （P 〈 0.05）. The 3 wk and 6 wk treated groups showed obvious decrease of α-SMA expression than that in the models, especially in the 6 wk treated group （P 〈 0.05） . CONCLUSION Cordyceps mycelium extract possesses remarkable effect against steatohepatitis and fatty hepatic fibrosis, with probable mechanism of the prevention from lipid peroxidat

关 键 词：肝硬化 实验性 脂质过氧化作用 大鼠 虫草菌丝 脂肪性肝炎 

分 类 号：R36[医药卫生—病理学] R286[医药卫生—基础医学]