HBME-1和p14^(ARF)抑癌基因在甲状腺肿瘤诊断与鉴别中的意义  

EXPRESSION OF HBME-1 AND p14^(ARF) IN PAPILLARY THYROID CARCINOMAS AND THEIR DIFFERENTIAL DIAGNOSES

作　　者：何春年[1] 赵焕芬[1] 陈士超[1] 翟金萍[1] 石卫东[1] 陈琛[1] 

机构地区：[1]河北省人民医院病理科,河北石家庄050051

出　　处：《河北医科大学学报》2008年第1期50-53,I0003,共5页Journal of Hebei Medical University

基　　金：河北省医学科学研究课题资助项目(06007)

摘　　要：目的探讨HBME-1和p14ARF抑癌基因蛋白在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中的表达状况;寻找更好的标记物,用于PTC与甲状腺滤泡状腺瘤(follicular adenomas,FA)和甲状腺良性乳头状病变(benign papillary lesions,BPL)等的鉴别诊断。方法以PTC为研究组,以FA和BPL作对照组。经成功自制组织芯片(tissue chips)和免疫组织化学SP方法,对HBME-1和p14ARF进行标记。结果PTC组,2种指标阳性表达率分别为94.0%和28.9%;FA组,2种指标阳性率分别为0.0%和75.0%;BPL组,2种指标阳性率分别为2.1%和77.5%。PTC组与FA组和BPL组比较均有显著性差异(P=0.000)。2种指标在鉴别诊断甲状腺良、恶性病变的灵敏度、特异度、准确度分别为94.0%和74.6%;95.7%和71.1%;95.1%和76.5%。结论PTC中标记2种指标,HBME-1高表达,p14ARF低表达。用于诊断PTC并与FA或BPL鉴别有实用意义。Objective To study the expression of HBME-1 and p14^ARF in papillary thyroid carcinoma（PTC） and to assess their usefulness as markers in the differential diagnosis of PTC, follicular adenomas（FA） and benign papillary lesions（BPL）. Methods Manual tissue chip and SP immunohistochemical stain were performed to detect the expression of HBME-1 and p14^ARF in PTC,FA and BPL. Results In PTC, positive rates of HBME-1 and p14ARE were 94.0% and 28.9%; in FA, positive rates were 0.0% and 75.0%; in BPL, positive rates were 2.1% and 77.5% ,respectively. The differences between PTC and FA and between PTC and BPL were both , statistically significant （ P = 0. 000）. The sensitivity of HBME-1 and p14^ARF was 94.0% and 74.6%; the specifity was 95.7% and 71.1%; accuracy was 95.1% and 76.5%, respectively. Conclusion High expression of HBME-1 and low expression of p14^ARF was bear out in PTC. So, conclude that HBME-1 and p14^ARF may be helpful in diagnosis and differential diagnosis of thyroid lesions.

关 键 词：甲状腺肿瘤 基因表达 免疫组织化学 

分 类 号：R736.1[医药卫生—肿瘤]