地塞米松对肾小球系膜细胞肿瘤坏死因子α产生和基因表达的影响  

Effect of dexamethasone on tumor necrosis factor alpha production and mRNA expression in glomerular mesangial cells

作　　者：于力[1,2] 杨霁云[1,2] 丁洁[1,2] 张英[1,2] 

机构地区：[1]北京医科大学第一医院儿科 [2]广州市第一人民医院儿科

出　　处：《中华儿科杂志》1997年第6期301-303,共3页Chinese Journal of Pediatrics

基　　金：卫生部科研基金;美国中华医学基金

摘　　要：为观察地塞米松对内毒素诱导的肾小球系膜细胞肿瘤坏死因子α（ＴＮＦα）的产生及其ｍＲＮＡ表达的影响，采用免疫学和分子生物学方法，对正常、内毒素诱导后及加入地塞米松的内毒素诱导后大鼠系膜细胞的ＴＮＦα水平及ＴＮＦαｍＲＮＡ表达进行了检测。结果：（１）ＴＮＦα在正常系膜细胞中分泌量极微，ＴＮＦαｍＲＮＡ的表达处于低水平；（２）内毒素诱导系膜细胞ＴＮＦα的产生及ｍＲＮＡ表达增加；（３）地塞米松对内毒素诱导的系膜细胞ＴＮＦα的产生及其ｍＲＮＡ表达有明显的抑制作用，呈剂量依赖关系。结论：地塞米松是系膜细胞产生细胞因子ＴＮＦα的较强抑制剂。The present study was undertaken to examine the effects of dexamethasone on the tumor necrosis factor alpha (TNFα) production and mRNA expression induced by endotoxin in cultured rat glomerular mesangial cells. We detected TNFα production and mRNA expression in normal glomerular mesangial cells, endotoxin induced glomerular mesangial cells and dexamethasone treated endotoxin induced glomerular mesangial cells by immunological and molecular biological methods. The results were as follows: (1) TNFα secretion and mRNA expression in normal mesangial cells were at low levels; (2) the protein production and mRNA expression of TNFα were increased by endotoxin inducement; (3) dexamethasone inhibited TNFα production and mRNA expression in mesangial cells induced by endotoxin in a dose dependent manner. We concluded that dexamethasone was an inhibitor for TNFα production and mRNA expression in mesangial cells.

关 键 词：地塞米松 肾小球膜 肿瘤坏死因子 肾小球肾炎 

分 类 号：R692.310.5[医药卫生—泌尿科学] R977.1[医药卫生—外科学]