不同牙周状况龈下菌斑中牙龈卟啉单胞菌和福赛斯坦纳菌的分布  

The distribution of Porphyromonas gingivalis,Tannerella forsythia in chinese periodontally healthy adults and chronic periodontitis patients

作　　者：杨禾[1] 赵蕾[1] 孟姝[1] 吴亚菲[1] 赵寰[1] 徐屹[1] 

机构地区：[1]四川大学华西口腔医学院牙周科,成都610041

出　　处：《现代口腔医学杂志》2008年第1期20-24,共5页Journal of Modern Stomatology

基　　金：十五国家科技攻关计划项目资助(No2004BA720A26)

摘　　要：目的分析中国牙周健康者和慢性牙周炎患者龈下菌斑中牙龈卟啉单胞菌和福赛斯坦纳菌的分布情况,探讨两种细菌在中国慢性牙周炎患者中的分布及其致病机理。方法收集65例牙周健康者、62例慢性牙周炎患者的龈下菌斑,采用16SrRNAPCR方法检测牙龈卟啉单胞菌和福赛斯坦纳菌的分布。结果牙周健康者牙龈卟啉单胞菌和福赛斯坦纳菌的检出率分别是21.6%、12.3%,慢性牙周炎患者病变位点两菌检出率分别为74.2%、58.1%,牙周炎健康位点两菌的检出率分别是22.6%、4.8%。牙周炎病变位点两种细菌的检出率均明显高于牙周健康者和牙周炎健康位点(P<0.001);福赛斯坦纳菌在牙周健康者中的检出率高于牙周炎健康位点(P<0.05);牙周健康者和牙周炎健康位点牙龈卟啉单胞菌的检出率没有差异。慢性牙周炎患者两菌联合检出率为51.6%。结论牙龈卟啉单胞菌、福赛斯坦纳菌以及两种细菌联合感染与牙周炎密切相关。Objective To investigate the distribution of P. gingivalis and T. forsythia in periodontally healthy adults and chronic periodontitis patients. Methods Subgingival plaque samples were collected from 65 periodontally healthy adults and 62 chronic periodontitis patients. P. gingivalis and T. forsythia were detected by 16S rRNA polymerase chain reaction. Results The prevalence ofP. gingivalis , T. forsythia differed significantly in periodontally healthy adults and patients with periodontitis （ P 〈 0. 001 ）. The prevalence of P. gingivalis and T. forsythia in periodontal healthy subjects was 21.6% and 12.3% respectively. The prevalence ofP. gingivalis , T. forsythia in diseased sites of patients with chronic periodontitis was 74.2% ,58.1%. The prevalence of these bacteria in disease sites were significantly higher than in healthy sites （22.6% ,4.8% ） （ P 〈 0.001 ）. P. gingivalis and T. forsythia detected in the same diseased site samples was 51.6%. Conclusion These results indicate a possible association between chronic periodontal disease and the presence of P. gingivalis and/or T. forsythia.

关 键 词：牙周炎 牙龈卟啉单胞菌 福赛斯坦纳菌 聚合酶链反应 

分 类 号：R781.4[医药卫生—口腔医学]