脂肪来源干细胞诱导分化为成牙本质样细胞的体外培养研究  

A study on differentiation of adipose-derived stem cells into odontoblast-like cells in vitro

作　　者：陈小红[1] 刘鲁川[1] 金岩[2] 邓蔓菁[1] 安建平[1] 方军[2] 孙雅娟[1] 

机构地区：[1]重庆第三军医大学大坪医院野战外科研究所口腔科,400042 [2]西安第四军医大学组织工程研发中心

出　　处：《实用口腔医学杂志》2008年第1期9-12,共4页Journal of Practical Stomatology

基　　金：第三军医大学科技创新工程(编号:2004);重庆市自然科学基金资助(编号:2005bb5307)

摘　　要：目的:探讨大鼠脂肪来源干细胞(ADSCs)向成牙本质样细胞分化的可能性。方法:SD仔4只,处死后取腹股沟处脂肪组织。采用细胞体外共培养的方法,在牙胚细胞条件培养液(TGC-CM)的诱导下,通过形态学观察、免疫组化、RT-PCR等方法,观察脂肪来源干细胞向成牙本质样细胞分化的过程。结果:大鼠脂肪来源干细胞在诱导培养基中生长良好。培养7d,在诱导组中细胞出现核极化,有很长的突起,细胞平行排列。抗牙本质涎蛋白(DSP)呈阳性反应。RT-PCR显示mRNA水平表达成牙本质细胞特异的牙本质涎磷蛋白(DSPP)和牙本质基质蛋白(DMP-1)。结论:含有多种细胞因子的TGC-CM能提供较好的微环境,诱导大鼠脂肪来源干细胞向成牙本质样细胞分化,能为组织工程牙体再生提供种子细胞来源和微环境选择。Objective ：To investigate the possibility of adipose-derived stem cells differentiating into odontoblast-like ceils. Methods： Adipose-derived stem ceils were isolated from four Sprague-Dawley rats and cultured in tooth germ ceils conditioned medium（TGC-CM））. Adipose-derived stem ceils were observed morphologically and immunohistochemically. Results：7 days after induction by TGC-CM, some ceils were tall column and polarized with long processes which tended to align in straight parallel lines. The ceils expressed dentin sialoprotein（DSP）. RT-PCR results showed the ceils expressed specific dentin sialophosphoprotein（DSPP） and dentin matrix protein-1（DMP-1）. Conclusion ：The results show that TGC-CM contains multiple cytokines which can create a odontogenic development microenvironment and induce the adipose-derived stem cells into odontoblast-like ceils.

关 键 词：牙胚细胞条件培养液 脂肪来源干细胞 成牙本质样细胞 分化 

分 类 号：Q813[生物学—生物工程]