IL-18在口腔鳞状细胞癌中的表达  

Expression of interleukin-18 in oral squamous cell carcinoma

作　　者：刘炜炜[1] 张茹慧[1] 韩艳非[2] 

机构地区：[1]长春吉林大学口腔医学院口腔颌面外科,130041 [2]吉林大学基础医学院免疫实验室

出　　处：《实用口腔医学杂志》2008年第1期107-110,共4页Journal of Practical Stomatology

摘　　要：目的:探讨白介素18在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达。方法:采用酶联免疫反应(ELISA)方法检测30例口腔鳞癌及10例正常人血清中IL-18的表达水平;免疫组织化学SP法检测36例口腔鳞癌组织和10例正常口腔黏膜组织中IL-18蛋白的表达。结果:口腔鳞癌患者血清中IL-18表达水平高于对照组(P<0.05)。中、低分化患者血清中IL-18水平明显高于高分化者(P<0.05);IL-18的水平随肿瘤浸润范围的扩大有逐渐升高的趋势,但无统计学意义(P>0.05)。癌组织中IL-18表达的阳性率低于正常的黏膜组织(P<0.05)。癌细胞分化程度越高,IL-18的表达越强(P<0.05),19例癌组织中IL-18弱表达或不表达,均是中、低分化者。血清中IL-18的表达量与癌组织中的阳性率呈反比。结论:IL-18在口腔鳞癌患者血清中表达明显升高,癌组织中表达下降,二者呈负相关,并且IL-18的表达与癌细胞的分化程度关系紧密,可作为判断口腔鳞癌病理分级的重要参考指标之一。Objective：To investigate the expression of interleukin-18 in oral squamous cell carcinoma. Methods： Expression of IL-18 in serum samples of 30 patients with OSCC and 10 healthy controls was determined by ELISA. Furthermore, immunohistochemical analysis was performed on tumor specimens from 36 patients with OSCC and 10 normal mucosal tissues. Results：Compared to healthy controls,the IL-18 in serum level in OSCC patients was significantly higher（ P 〈 0.05 ）. Significantly higher concentrations of IL-18 could be observed in serum from patients in moderately or poorly differentiated OSCC as compared to well-differentiated OSCC （P 〈 0.05 ）. As the tumor invasion progressed, serum IL-18 levels tended to increase, although there was no statistically significance（ P 〉0.05 ）. The expression of IL-18 in OSCC tissue specimens was significantly lower compared to healthy subjects（ P 〈 0.05 ）. The higher the cancer differentiated, the higher the IL-18 expression level is observed（ P 〈 0.05 ）. IL-18 expression was not found in 19 middle or poorly differentiated OSCC specimens. Conclusion： In the case of OSCC, IL-18 level is increased in serum and decreased in tumor tissues, which indicats negative correlation. It is correlated with differentiation of cancer cell and might be one of the useful patho-grading marker of OSCC.

关 键 词：白细胞介素-18 口腔 鳞状细胞癌 酶联免疫吸附试验 

分 类 号：R739.8[医药卫生—肿瘤]