包载反义MRP重组腺病毒微球肝动脉注射治疗肝癌的实验研究  

Hepatic arterial injection of recombinant adenovirus microspheres carrying antisense multidrug resistance-associated protein gene for hepatocellular cancer in rats

作　　者：王晓波[1] 余少鸿[1] 严律南[2] 陈永兵[2] 

机构地区：[1]重庆市涪陵中心医院肝胆外科,重庆涪陵408000 [2]四川大学华西医院普通外科,四川成都610041

出　　处：《中国普通外科杂志》2008年第1期13-15,共3页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(30170925)

摘　　要：目的探讨包载反义MRP重组腺病毒微球逆转肝癌的效果。方法设无处理组、生理盐水组、空白微球组as-MRP rAdV组,采用携带as-MRP rAdV PELA制备的微球经肝动脉注射组等5组,观察其靶向性治疗效果。结果rAdV微球组肿瘤体积及生长率显著小于其他4组(均P<0.05),平均生存时间显著延长(均P<0.05),可见肝癌组织rAdv荧光强表达。结论rAdV微球可显著缩小肝脏肿瘤体积,抑制肿瘤生长。Objective To evaluate recombinant adenovirus microspheres encapsulated with antisense MRP （as-mrp） on reversion of hepatocellular carcinoma. Methods The rats were divided randomly into 5 groups：control group; hepatic arterial injection of normal saline group, microspheres carrying no viruses group, rAdV carrying as-mrp group, and microspheres with encapsulation of as-mrp rAdV （MER） group. The theraputic effect were observed. Results The tumor growth inhibition and the mean life time in MER group were superior to than of other 4 groups （ P 〈 0. 05 ） . The tumor tissue were observed to have fluorescence intensity, but was negative in other tissues. Conclusions Recombinant adenovirus microspheres can effectively inhibit HCC.

关 键 词：癌 肝细胞/治疗 MRP 反义 腺病毒 重组 微球 肝动脉注射 

分 类 号：R735.7[医药卫生—肿瘤]