肿瘤细胞节律基因对其药物敏感性的影响  

Effects of Circadian Gene of Tumor Cells on Its Drug Sensitivity

作　　者：段志青[1] 要然[1] 薛建新[1] 赵京卉[1] 朱小云[1] 汪宇辉[1] 刘延友[1] 王正荣[1] 

机构地区：[1]四川大学华西基础医学与法医学院,四川成都610041

出　　处：《航天医学与医学工程》2008年第1期76-78,共3页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助(30470623;30470684)

摘　　要：目的通过体外实验诱导节律基因表达,探讨肿瘤细胞节律基因对其药物敏感性的影响。方法采用12-十四酸佛波酯-13-乙酸盐(PMA)诱导体外培养的EMT6乳腺癌细胞节律基因的表达;在不同时间点,以RT-PCR检测节律基因的代表mPer1基因的表达水平。在mPer1基因表达的高峰与低谷分别给予阿霉素(ADM)处理。采用二甲基噻唑二苯基四唑嗅蓝(MTT)法和流式细胞术检测ADM对EMT6细胞增殖的影响。结果PMA诱导后,EMT6细胞的mPer1基因呈近日节律。在mPer1基因表达高峰时,ADM对肿瘤细胞的抑制率高于其在低谷的抑制率。结论肿瘤细胞节律基因能够影响其对药物的敏感性。Objective To investigate the effects of rhythm gene of tumor cells on its drug sensitivity through induced the circadian genes expression in vitro, Methods The circadian genes expression of cultured EMT6 mouse mammary carcinoma cells was induced with phorbol 12-myristate 13-acetate （PMA） in vitro and examined the expression of mPerl genes considered as the represent of rhythm genes at the different time with RT-PCR, EMT6 cells were dealt with adriamycin （ADM, 2 μg/ml） at the maximum and the minimum of mPerl genes respectively. The effects of ADM on proliferation of EMT6 cells was detected with 3-（4, 5-dimethylthiazolyl-2）-2, 5-diphenyltetrazolium bromide （MTT） and flow cytometry （FCM）. Results It was showed that circadian oscillation of mPerl exis- ted in EMT6 cells induced with PMA and that the inhibition ratio of ADM to tumor cells at the maximum of mPerl gene was higher than that at the minimum of it. Conclusion The rhythm gene of tumor cells has effect on its drug sensitivity.

关 键 词：近日节律 Perl基因 EMT6细胞 阿霉素 细胞增殖 

分 类 号：Q789[生物学—分子生物学]