EPA、DHA对脂多糖诱导的大鼠系膜细胞增殖及凋亡的影响  

Effects of EPA and DHA on the proliferation and apoptosis of glomerular mesangial cells induced by lipopolysaccharide

作　　者：耿文静[1] 柳方娥[1] 焦波[2] 程艳娜[2] 

机构地区：[1]山东大学第二医院儿科,济南250012 [2]山东大学药学院药理教研室,济南250012

出　　处：《山东大学学报（医学版）》2008年第1期57-59,共3页Journal of Shandong University:Health Sciences

基　　金：山东省优秀中青年科学家奖励基金资助课题(2005BS02019)

摘　　要：目的观察二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对正常及在脂多糖(LPS)诱导下的肾小球系膜细胞(GMCs)增殖和凋亡的影响。方法EPA和DHA各设两个终浓度(10μmol/L和100μmol/L),LPS终浓度为10μg/mL,培养时间为24、48和72 h,采用MTT法测定细胞增殖;EPA和DHA终浓度为100μmol/L,培养48 h后,采用双染色流式细胞术检测细胞凋亡。结果EPA和DHA对正常GMCs无明显影响;对LPS诱导的GMCs增殖有明显抑制作用(P<0.01),在同等浓度下,DHA的作用强于EPA(P<0.01);EPA、DHA可明显促进GMCs凋亡。结论EPA和DHA明显抑制LPS诱导的GMCs增殖并促进其凋亡。Objective To observe the effects of EPA and DHA on the proliferation and apoptosis of glomerular mesangial cells （GMCs） induced by lipopolysaccharide（LPS）. Methods The GMCs were incubed with EPA and DHA at concentrations of 10 μmol/L and 100 μmol/L for 24, 48 and 72 hs. The proliferation of GMCs was determined by MTT. The apoptosis of GMCs was determined by flow cytometry. Results Both EPA and DHA had no effect on the proliferation of normal GMCs. The proliferation of GMCs induced by LPS was significantly inhibited. DHA was more effective than EPA at the same concentration. The apoptosis of GMCs was promoted by EPA and DHA（ 100 μmol/L）, Conclusion EPA and DHA could inhibit the proliferation and promote the apoptosis of GMCs induced by LPS.

关 键 词：二十碳五烯酸 二十二碳六烯酸 系膜细胞 增殖 

分 类 号：R692.6[医药卫生—泌尿科学]