重组人p53腺病毒联合肿瘤坏死因子对乳腺癌Ca761细胞的抑制作用  

Experimental study of recombinant adenoviral-mediated p53 gene transfer in combination of TNF in breast cancer of mouse model

作　　者：张晓明 戚晓东[2] 何时知 王宇[2] 

机构地区：[1]山东临沂市第二人民医院普外科,山东临沂276000 [2]首都医科大学附属北京同仁医院肿瘤中心,北京100730

出　　处：《现代肿瘤医学》2008年第2期182-185,共4页Journal of Modern Oncology

摘　　要：目的:探讨重组人p53腺病毒注射液(rAd/p53)联合肿瘤坏死因子(TNF)对荷瘤小鼠体内外的乳腺癌细胞Ca761的抑制效果。方法:MTT法,流式细胞术检测细胞凋亡率,观察rAd/p53和TNF对乳腺癌细胞的体外抑制作用及促凋亡作用。建立乳腺癌Ca761细胞小鼠移植瘤模型,瘤内分别注射rAd/p53和TNF及两药联合,观察对肿瘤生长的影响,并对肿瘤组织进行病理学和免疫组化检查。结果:rAd/p53和TNF体内外均可显著抑制乳腺癌Ca761细胞的生长,可以促进乳腺癌细胞Ca761株的凋亡。两药联合对肿瘤细胞具有协同杀伤作用。结论:重组人p53腺病毒注射液(rAd/p53)治疗Ca761乳腺癌细胞移植瘤是有效的,TNF也可抑制其生长,rAd/p53和TNF联合应用有协同作用,为临床乳腺癌的综合治疗提供了依据。Objective：To explore the effect of a recombinant adenoviral -mediated p53 gene transfer in combination of TNF to the mice transferred with breast cancer Ca761. Methods： The cell growth inhibition and apoptosis of rAd/p53 and TNF were detected by MTT and flow cytometry. The mice model bearing breast cancer was established using Ca761 breast cancer cell. rAd/p53 , TNF and combination of two drugs were injected into the tumor. The growth of tumor was observed , and detected by pathologic and immunohistochemical S - P method. Results： rAd/p53 and TNF could inhibit the growth of breast cancer in vitro and vivo. rAd/p53 in combination of TNF could signigicantly inhibit the growth of tumor. Conclusion：It is feasible for a recombinant adenoviral -mediated p53 gene therapy to treat breast cancer, and it can offer the proof for breast cancer combined therapy. TNF can also inhibit the growth of breast carcinoma. The combined treatment of rAd/p53 and TNF is better than either treatment alone for breast cancer.

关 键 词：rAd/p53 TNF 乳腺癌 P53基因 疗效 

分 类 号：R73-362[医药卫生—肿瘤]