原发骨肿瘤端粒酶活性的检测及临床意义  

The detection and clinical significance of telomerase activity in primary bone tumor

作　　者：郑勇[1] 李光辉[2] 陶德定[3] 李锋[2] 陈超[2] 夏仁云[2] 

机构地区：[1]咸宁医学院附属第一医院骨科,湖北咸宁437100 [2]华中科技大学同济医学院附属同济医院骨科,湖北武汉430030 [3]华中科技大学同济医学院附属同济医院分子医学中心,湖北武汉430030

出　　处：《现代肿瘤医学》2008年第2期278-279,共2页Journal of Modern Oncology

摘　　要：目的:探讨端粒酶在原发骨肿瘤组织中的活性表达及临床意义。方法:采用TRAP-ELISA法对21例骨肉瘤、11例骨巨细胞瘤、9例软骨肉瘤、3例骨恶性纤维组织细胞瘤、1例脊索瘤、11例骨软骨瘤、5例骨样骨瘤、4例非骨化性纤维瘤、6例孤立性骨囊肿及7例正常骨组织进行端粒酶活性检测。结果:45例原发恶性骨肿瘤中有38例显示端粒酶活性,阳性率为84.4%,26例良性病变组织中只有2例骨软骨瘤具端粒酶活性,7例正常组织端粒酶表达均为阴性。结论:恶性骨肿瘤组织中端粒酶活性明显高于良性病变和正常组织,有可能成为恶性骨肿瘤的一种潜在性生化标志物和鉴别诊断的指标。Objective：To investigate the expression of telomerase in primary bone tumor and its clinical significance . Methods： The telomerase activity was detected in 45 samples of malignant bone tumor , 26 samples of benign bone tumor or tumor - like lesion and 7 samples of normal tissues by using TRAP - ELISA . Results： Telomerase activity was found in 38 of 45 samples of malignant bone tumor , while 2 of 26 samples of benign bone tumor or tumor - like lesion was detected , and not expressed in 7 samples of normal tissues . Conclusion： Telomerase activity of malignant bone tumor was significantly higher than that of benign or normal tissues , it may be a potential biochemical marker for malignant bone tumor and helpful for differential diagnosis between malignant and benign bone tumor.

关 键 词：骨肿瘤 端粒酶 检测 

分 类 号：R738.1[医药卫生—肿瘤]