7TD1细胞及其克隆对IL-6的反应性和依赖性  

Reactivity and dependence of 7TD1 cell and its clone on IL 6

作　　者：巩新[1] 边疆[1] 薛冲[1] 陶好霞[1] 孙澎[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《军事医学科学院院刊》1997年第3期181-184,共4页Bulletin of the Academy of Military Medical Sciences

摘　　要：比较了本院基础医学研究所和北京医科大学提供的两株７ＴＤ１细胞。选择北京医科大学提供的细胞株进行克隆，筛选出对ＩＬ－６增殖反应性和依赖性较强的３株细胞（７ＴＤ１ＭＣ１、７ＴＤ１ＭＣ４、７ＴＤ１ＭＣ５）。其中７ＴＤ１ＭＣ５细胞的反应曲线呈典型的“倒Ｓ”形，对ＩＬ－６的反应性和依赖性很好。ＭＴＴ比色法显示了与３Ｈ－ＴｄＲ掺入法一致的结果，采用７ＴＤ１ＭＣ５细胞及ＭＴＴ比色法测定ＩＬ－６生物活性，可减少测试的费用和避免放射性污染。确定了培养基中ＩＬ－６的最适浓度（２ｎｇ／ｍｌ）。细胞传代培养和冻存６个月。Two 7TD1 cell lines provided by Institute of Basic Medical Sciences and Beijing Medical University were compared. Cloning with the cell line from Beijing Medical University, three cell strains (7TD1 MC1,7TD1 MC4 and 7TD1 MC5) which showed strong growth reactivity and dependence on IL 6 were screened. One of them, 7TD1 MC5, revealed reaction curve of typical “reversed S”, representing good reactivity and dependence on IL 6. Using 7TD1 MC5 cell and MTT method to detect biological activity of IL 6, the results obtained corresponded to those obtained with 3H TdR method while the cost was reduced and radioactive pollution was avoided. The optimal concentration of IL 6 in medium was determined to be 2 ng/ml. No obvious change in of reactivity and dependence of 7TD1 MC5 cell on IL 6 was observed after 6 months subculture and cryostorage.

关 键 词：7TD1细胞 克隆 白细胞介素6 反应性 依赖性 

分 类 号：R392.12[医药卫生—免疫学] R394.8[医药卫生—基础医学]