亚低温处理大鼠脑缺血再灌注损伤后谷氨酸载体EAAC1mRNA表达和损伤神经细胞凋亡的变化  

Changes of the glutamate transporter EAAC1mRNA expression and injuried nerve cell apoptosis in rat with cerebral ischemia/reperfusion injury treated by mild hypothermia

作　　者：吴国祥[1] 李承晏[1] 刘春英[1] 邹小丽[1] 夏军[2] 

机构地区：[1]武汉大学人民医院神经内科,430060 [2]武汉大学人民医院康复医学科,430060

出　　处：《中华物理医学与康复杂志》2007年第12期797-799,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的探讨亚低温对大鼠脑缺血再灌注损伤后谷氨酸载体兴奋性氨基酸载体(EAAC) 1mRNA表达和损伤神经细胞凋亡的影响。方法采用大鼠局灶性脑缺血再灌注损伤模型,大脑中动脉阻塞30min,再灌注损伤90min,用原位杂交法和原位缺口末端标记法分别检测假手术组、对照组和亚低温组的谷氨酸载体EAAC1mRNA表达阳性率和凋亡细胞百分率。结果与对照组比较,假手术组的EAAC1mRNA表达阳性率和凋亡细胞百分率均明显降低(P<0.05和P<0.01),而亚低温组EAAC1mRNA表达阳性率显著升高(P<0.05),但凋亡细胞百分率明显下降(P<0.05)。结论亚低温的抗脑缺血再灌注损伤后损伤神经细胞凋亡作用可能与其上调EAAC1mRNA表达、诱导EAAC1合成及增加谷氨酸的摄取有关。Objective To investigate the changes in the glutamate transporter EEAClmRNA expression and the injured nerve cell apoptosis after rat cerebral ischemia/reperfusion injury is treated with mild hypothermia. Methods The middle cerebral arteries （MCA） of Sprague-Dawley rats were occluded for 30 minutes, and reperfused for 90 minutes. Using DIG-labeled CRNA probe and TUNEL, the positive rate of glutamate transporter EEAC1 mRNA expression and apoptotic cell rate were determined in the sham-operated group, the control group and the mild hypothermia group, respectively. Results The positive rate of EEAClmRNA expression and apoptotic cell rate were significantly lower in sham-operated group than those in the control group （ P 〈 0.05 ,P 〈 0.01 ）. The posi- tive rate of the EEAClmRNA expression was significantly higher and the apoptotic cell rate was significantly lower in mild hypothermia group than those in the control group（ P 〈 0.05 ）. Conclusion That the mild hypothermia decreases the injured nerve cell apoptosis might be related to the increased EEAC1 mRNA expression, the induced synthesis of EEAC1 and the increased uptake of glutamate.

关 键 词：亚低温 脑缺血再灌注损伤 兴奋性氨基酸载体1mRNA 凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]