低温海水浸泡协同力竭性游泳致应激性溃疡的大鼠模型建立  

Establishment of rat stress ulcer model induced by cold sea water-immersion and exhausted swimming

作　　者：骆益宙[1] 缪冬梅 傅继华[3] 

机构地区：[1]解放军第454医院肿瘤科,江苏南京210002 [2]南京港口医院内科,江苏南京210011 [3]中国药科大学生理教研室,江苏南京210009

出　　处：《东南国防医药》2007年第6期420-421,共2页Military Medical Journal of Southeast China

基　　金：南京军区医药卫生科研项目(06MA71)

摘　　要：目的通过低温海水浸泡、力竭性游泳刺激,建立应激性溃疡的动物模型。方法Wistar雄性大鼠56只分4组,A、B、C组各16只,D组8只。A组0℃海水浸泡同时给予疲劳性游泳刺激;B组单纯0℃海水浸泡;C组力竭性游泳刺激;D组为正常对照组。通过观察胃粘膜溃疡的发生率和Guth法评价溃疡分数。溃疡局部胃组织进行病理学检查。结果A组发生应激性溃疡12只(75.0%),B组发生应激性溃疡7只(43.8%),C组发生应激性溃疡6只(37.5%),D组未发现溃疡。A组应激性溃疡发生率高于B、C组(P<0.01),B、C组之间无显著性差异(P>0.05)。溃疡分数A组(33.5±1.2)、B组(23.1±11.6)、C组(27.7±12.2)、D组(1.0±0.2),A组和B、C组之间存在显著性差异(P<0.01),B、C组之间无显著性差异(P>0.05)。结论低温海水浸泡加力竭性游泳刺激可有效诱导应激性溃疡,是理想的动物模型。Objective To establish a stress ulcer model induced by cold sea water-immersion and exhausted swimming. Methods 56 male Wister rats were divided into 4 groups,16 rats was included in A,B and C group,the other 8 rats in group D. Rats in group A were immersed in 0℃ cold sea water and exhausted by swimming,in group B were immersed in 0℃ cold sea water,in group C were exhausted by swimming,group D as control. The rate of gastric ulcer in experimental rats and ulcer index by Guth method were calculated. The ulcer lesion was collected for further pathological examination. Results Stress ulcers occurred in i group A（75.0% ） ,B（43.8% ） and C（37.5% ） ,no stress ulcer was obversed in group D. The occurrence rate of stress ulcer in group A was higher than group B and C （P〈0. 01） ,no difference was found between group B and C（P〉0.05）. Ulcer index in group A （33. 5±1.2） was higher than group B（23.1±11.6） and C（27.7±12.2）（P〈0. 01）,no difference was found between group B and C （P〉0. 05）. Conclusion Cold sea water immersion and exhausted swimming induce stress ulcer effectively,an ideal model of stress ulcer is estabished.

关 键 词：应激 溃疡病 动物模型 

分 类 号：R573.1[医药卫生—消化系统]