三株新型发酵产氢菌的分离和不同发酵条件下产氢性能的比较  

Isolation of Three Novel Fermentative H2-Producing Bacteria and Comparison of Their H2-Producing Ability under Different Conditions

作　　者：陈瑛[1] 任南琪[1] 程瑶[1] 李永峰[1] 张大维[2] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090 [2]哈尔滨师范大学生物系,哈尔滨150080

出　　处：《科技通报》2008年第1期133-137,共5页Bulletin of Science and Technology

基　　金：国家杰出青年科学基金资助项目(50125823);国家863项目(2006AA05Z109)

摘　　要：氢能是理想的清洁能源,生物制氢技术的研究和开发已成为当前一个研究热点。许多细菌在厌氧条件下发酵复杂的有机化合物,同时产生H2。本文选择了来自同一活性污泥样品的3株有较高产氢能力的发酵细菌,采用生化和电镜技术对其形态学特征进行了观察比较,并进一步比较研究了在不同pH值条件下,它们产氢量、细菌生长量、液相发酵产物组成的变化和差异。获得了三株产氢菌的单位体积培养基最大累积产氢量,提出了纯培养生物制氢的最佳条件,为混合培养生物制氢强化工艺提供参考。Hydrogen gas is perfect and clean energy. The study of biohydrogen producing techniques has been a hot spot today. Under anaerobic condition,many bacteria can ferment complex organic compound to generate hydrogen gas. In this paper, three novel fermentative hydrogen producing bacteria isolated from an active sludge sample were chose to compare and observe their morphology characteristics by methods of biochemistry and electronic microscope. Furthermore, the variations and differences of cumulative production of hydrogen gas,density of bacteria and end liquid products were studied under different pH conditions. Their maximum cumulative H2-producing quantities of unit volume substrate were achieved and the optimum of biohydrogen process by pure culture was suggested to offer references for intensifying biohydrogen process by mixed culture.

关 键 词：生物制氢 发酵条件 产氢微生物 

分 类 号：X703[环境科学与工程—环境工程]