3株放线菌对草莓的促生作用及对PPO活性的影响  

The Growth Promoting Effect and Induced Endurance of Three Actinomyces Strains to Strawberry

作　　者：许英俊[1] 薛泉宏[2] 邢胜利 周永强[1] 张晓鹿[1] 郭志英[2] 杨斌[1] 林超峰[1] 孙敬祖[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学资源与环境学院,陕西杨凌712100 [3]陕西省农技推广总站,陕西西安710003

出　　处：《西北农业学报》2008年第1期129-136,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省科技攻关项目(2004K02-G7)

摘　　要：采用琼脂块法、拌土育苗和蘸根盆栽生物试验及PPO活性测定法研究供试放线菌对病原菌的皿内拮抗作用、对草每的促生作用及诱导抗性。结果表明：①供试放线菌对5种草莓土传病害病原菌有明显的抑制作用。Act11对5种病原菌均有较强的抑制作用；Act12对尖孢镰刀菌外的4种病原菌有拮抗作用，Act1对尖孢镰刀菌和草莓疫霉有拮抗作用。②生防菌剂接种促生作用明显，对根系生长的刺激效应尤为显著。在拌土接种育苗处理中，草莓根系及茎叶重量分别较对照提高122．4％～265．6％和53．6％～64．4％。③生防菌接种后可提高草莓新叶和根系PPO活性，产生诱导抗性；根系PPO活性增幅大于叶片。在拌土育苗和蘸根盆栽处理中，草莓新叶PPO活性较对照提高幅度均值分别为5．9％和16．9％；草莓粗根PPO活性提高幅度均值分别为21．4％和35．0％，细根PPO活性提高幅度均值分别为31．3％和26．3％。由此得出结论：供试生防菌在皿内能抑制草莓常见土传病病原菌生长；在拌土育苗和蘸根盆栽条件下均能显著促进草莓根系发育及茎叶生长，并使草莓产生诱导抗性。Measure the inhibition by agar piece method. After puddling treatment raise seedlings and dipping root pot culture test, get the growth promoting effect to the strawberry and measure the strawberry leaves and root＇s PPO activity, so as to get the induced endurance. The results showed that： （1）The three actinomyces strains all have transparent inhibition to the five pathogenetic fungi. Act11 has strong effect to the five disease germs. Act12 has inhibitory action to four disease except Fusarium oxysporun. Actl has the same effect on both Fusarium and P. fragaride Hickm. （2）The three actinomyces strains all have transparent growth promoting effect after the inoculation, especially to the root of the strawberry. The root weight rising by 122.4% to 265.6% over check test and the leaves-stem reach to 53. 6% to 64.4%. （3）The three actinomyces strains can promote the leaves and root＇s PPO activity, produce the induced endurance of the strawberry after the inoculation. The effect to the root＇s PPO activity is stronger than the leaves＇. After puddling treatment raise seedings and dipping root pot culture test, the three actinomyces strains can improve the strawberry néw leaves＇ PPO activity in 5.9% and 16.9% ,improve the strawberry thick root＇s PPO activity in 21.4% and 35. 0% ,as well as thin root＇s activity in 31.3% and 26.3%. The three actinomyces strains all have inhibition to the five strawberry pathogenetic fungi. After puddling treatment raise seedlings and dipping root pot culture test, they can improve each part＇s biomass of the strawberry plant and make the strawberry produce induced endurance.

关 键 词：草莓 生物防治 放线菌 拮抗 生物量 PPO活性 诱导抗性 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]