高比活度[α-^(32)P]ATP的酶法标记  

THE ENZYMATIC PREPARATION OF [α 32 P]ATP WITH HIGH SPECIFIC ACTIVITY

作　　者：吴冯波[1] 王翌善[1] 仪明光 

机构地区：[1]中国原子能科学研究院同位素研究所

出　　处：《核化学与放射化学》1997年第1期46-50,共5页Journal of Nuclear and Radiochemistry

摘　　要：以［γ－３２Ｐ］ＧＴＰ作磷酸供体通过四步酶反应制备了高比活度（＞１４８ＰＢｑ／ｍｏｌ）的［α－３２Ｐ］ＡＴＰ。用高效液相色谱法（ＨＰＬＣ）测定了［α－３２Ｐ］ＡＴＰ比活度，并对影响［α－３２Ｐ］ＡＴＰ比活度的诸因素作了分析。A modified procedure for preparing [α 32 P]ATP is described with which [γ 32 P]GTP is used as phosphate donor instead of [γ 32 P]ATP.The specific activity of the resulted[α 32 P]ATP in our experiments is raised greatly as to 148 PBq/mol or more,without decreasing the yield of [α 32 P]ATP which is over 93% based on 32 Pi.The specific activity is measured by HPLC and the factors that affect the specific activity of [α 32 P]ATP are discussed.

关 键 词：酶法 标记 分子生物学 GTP ATP 比活度 磷32 

分 类 号：Q7[生物学—分子生物学]