SAF-Tween80-β-CD荧光熄灭法测定微量钼的研究  

Fluorescence quenching method for determination of trace molybdenum with salicylfluorone in the presence of Tween80 and β-CD

作　　者：李英杰[1] 陈世界[1] 高立娣[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《分析试验室》2008年第1期31-33,共3页Chinese Journal of Analysis Laboratory

基　　金：黑龙江省科技攻关(GC02A313)项目资助

摘　　要：在HCl介质中，吐温80（Tween80）和β-环糊精（β-CD）存在下，钼与水杨基荧光酮（SAF）形成复杂配合物使荧光熄灭，由此建立了测定微量钼的荧光熄灭新方法。该体系的最大激发波长λex=455n／n，最大发射波长λcm=522n／n。Mo（Ⅵ）量在0～0．12μg／mL范围荧光熄灭程度（AF）与Mo（Ⅵ）质量浓度呈线性关系，线性回归方程为△F=31．839ρ（μg／mL）＋29．726，相关系数R^2=0．9996，检出限为1．53μg／L。该方法灵敏度高，选择性好，用于测定水样中的微量钼，回收率在99．4％～100．8％之间。A new fluorescence quenching method for the determination of trace molybdenum is proposed in hydrochloric acid medium and in the presence of Tween80 and β-CD, and a ternary complex is formed by Mo（ Ⅵ ） and Salicylfluorune leading to fluorescence quenching. The maximum excitation and emission wavelengths are 455 nm and 522 nm, respectively. The linear range is 0.0- 3.0μg/25 mL. The linear regression equation is AF = 31.839c （μg/25 mL） ＋ 29. 726, The correlation coefficient is R2 = 0. 9996, The detection limit is 1.53μg/L. The method is selective and highly sensitive to molybdenum. The method has been applied to the direct determination of trace Mo （ Ⅵ ） in water samples with satisfactory results.

关 键 词：荧光熄灭 吐温80 水杨基荧光酮 β-环糊精 钼 

分 类 号：O657.3[理学—分析化学]