Bt棉不同种植年限土壤中Bt基因及其蛋白的残留测定  

Detection for Persistence of Bt Gene and Bt Insecticidal Proteins in Soil after Multiple Years of Bt Cotton Planting

作　　者：沈平[1,2] 张永军[1] 陈洋[1] 吴孔明[1] 彭于发[1] 郭予元[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094 [2]农业部科技发展中心,北京100026

出　　处：《棉花学报》2008年第1期79-80,F0003,共3页Cotton Science

基　　金：国家重点基础研究发展规划项目(001CB109004)

摘　　要：从16个多年种植Bt棉花的田块抽取土壤样本。采用PCR和ELISA方法测定了土壤试样中Bt基因序列片段和杀虫蛋白的残留和累积效应。结果表明,以土壤总DNA为模板不能扩增出棉花内标准基因和cry1A基因预期大小片段。连续种植Bt棉花可以在土壤中低剂量残留Bt杀虫蛋白,但不会导致Bt毒蛋白在土壤中累积。Soil samples were collected from within sixteen fields where insect-resistant transgenic cotton encoding the crylA gene had been grown and subsequently incorporated into soil by post-harvest tillage for multiple years. The crylA gene sequence fragments and Bt insecticidal protein in these samples （collected after the last season tillage） were explored using PCR amplification and enzyme-linked immunosorbent assay （ELISA） method, respectively. Soil total DNA could be gotten in high quality, but we could not get any PCR amplification result of cotton internal standard gene or crylA gene. The results from ELISA indicated that repeated agricultural using of transgenic Bt cotton expressing CrylA insecticidal protein could persist extremely low levels of Bt protein in soil. However, the persistence of Bt protein in soil did not result in accumulation by post-harvest tillage in multiple seasons.

关 键 词：BT棉花 土壤 BT杀虫蛋白 残留 

分 类 号：S562[农业科学—作物学]