仫佬族15个短串联重复序列的遗传分析  

Genetic analysis of Mulao nationality using 15 short tandem repeats

作　　者：徐林[1] 邓琼英[1] 李松峰[1] 周丽宁[1] 龚继春[1] 魏博源[1] 

机构地区：[1]广西医科大学人体解剖学教研室,南宁530021

出　　处：《中华医学遗传学杂志》2008年第1期96-100,共5页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金（30260044）

摘　　要：目的了解广西仫佬族15个常染色体短串联重复序列（ D2S1338, D3S1358, D5S818, D7S820,D8S1179,D13S317 ,D16S539,D18S51 ,D19S433,D21S11, CSF1PO,TPOX, THO1 ,vWA, FGA）的遗传多态性，探讨其与10个群体间的遗传关系。方法采用聚合酶链反应．短串联重复序列（PCR-short tandem repeat, PCR-STR）及基因扫描识别技术，研究广西仫佬族183名无关个体15个常染色体STR位点的等位基因频率的分布特点，然后计算11个群体间的遗传距离，用Ndghbo-Joining法构建了系统发生树，并结合有关资料分析了它们之间的遗传关系。结果（1）15个STR位点共检出136个等位基因，422个基因型，等位基因频率分布在0．0027～0．5243之间；平均杂合度为0．7632，个体识别力除TPOX位点外均大于0．8，累积个体识别力大于0．9999999999，累积非父排除率大于0．9999984698。（2）广西仫佬族与广西其它几个少数民族之间的遗传距离近，与汉族各群体、维吾尔族群体的遗传距离远。结论（1）广西仫佬族15个STR位点除TPOX位点外均具有高度遗传多态性，是群体遗传学研究和法医学鉴定的可选位点。（2）仫佬族与广西各少数民族之间的亲缘关系密切于与各地汉族之间的亲缘关系。Objective To study the genetic polymorphism of 15 short tandem repeats （STR） （D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S133, D21S11, CSF1P O, TPOX, TH01, vWA, FGA） in Mulao nationality of Guangxi province, and to explore genetic relationship between Mulao nationality and other 10 nationalies. Methods The allelic frequencies and the genotype of 15 STR loci were generated from 183 unrelated individuals in Mulao nationality and other 10 nationalities of Guangxi by PCR-STR and genescan. Phylogenetic tree were constracted neighbor-Joining method. Results There were 136 STR alleles and 422 genotypes in the 15 STR of Mulao nationality, with its allele frequencies ranging from 0.0027 to 0. 5243. The average heterozygosity was 0. 7632, the accumulative discrimination power was more than 0.999 999 999 9, and the probability of paternity exclusion was more than 0.999 998 469 8. The genetic distances between Mulao nationality and other minority of Guangxi were much closer than those between Mulao nationality and Han nationality and Uighur nationlity. Conclusion The 15 STR loci of Mulao nationality in Guangxi possesses the characteristics of high genetic diversity, except the TPOX locus. They can be employed in group genetic investigation, individual and paternity test in forensic medicine. The genetic distances between Mulao nationality and other minority of Guangxi are more closer than those between Mulao nationality and Han nationality and Uighur nationality.

关 键 词：短串联重复序列 遗传多态性 遗传距离 仫佬族 

分 类 号：R394[医药卫生—医学遗传学] R516[医药卫生—基础医学]