有氧运动对C57BL／6小鼠骨骼肌PPARδ表达和肌纤维类型的影响  

Effects of Endurance Training on PPARδ Expression and Skeletal Muscle Fiber Type in C57BL/6 Mice

作　　者：苏丽[1] 姜宁[1] 张玥[1] 牛燕媚[1] 苑红[1] 席翼[1] 张勇[1] 傅力[1] 

机构地区：[1]天津体育学院运动医学研究所,运动人体科学系,天津300381

出　　处：《中国运动医学杂志》2008年第1期27-31,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金(No30570898);天津市重大攻关项目(05YFGDSF02100)共同资助

摘　　要：目的:研究有氧耐力运动对骨骼肌PPARδ的表达及由此对肌纤维类型的影响,探讨骨骼肌对耐力训练产生适应性反应的生物学机制。方法:本研究选用雄性C57BL/6小鼠90只,分别随机分为3周(TC)、6周(SC)、9周(NC)对照组和3周(TE)、6周(SE)、9周(NE)运动组,建立无负重游泳训练模型,采用Northernblot、Westernblot,免疫荧光和mATPase染色分析各组骨骼肌PPARδ的表达及肌纤维类型的变化。结果:有氧运动各组骨骼肌PPARδ转录和翻译水平与各自对照组相比显著提高。mATPase染色结果表明各运动组运动后腓肠肌纤维类型百分比无显著改变。结论:有氧耐力运动可诱导骨骼肌PPARδmRNA和蛋白表达增强,但并未导致骨骼肌纤维类型的显著变化,提示PPARδ可能在骨骼肌对运动训练的适应性过程中发挥重要作用,而单纯运动并不能诱导肌纤维类型转变的发生,其可能是细胞内代谢和外界干预因素共同作用的结果。Objective To explore the effect of aerobic endurance exercise on muscle PPAR8 expression and its potential influence on skeletal muscle fiber type. Methods Male C57BL/6 mice （6 week old） were randomly divided into six groups： three control groups of 3-week control （TC）, 6-week control （SC） and 9-week control （NC）, and three exercise groups of 3-week swimming （TE）, 6-week swimming （SE） and 9-week swimming （NE）. The mice in all exercise groups swam without extra loading. The mice were sacrificed according to the schedule, and tissues were removed. Northern blot, Western blot and mATPase staining were applied to analyze the effects of endurance training on skeletal muscle PPAR~ mRNA, protein expression and their relationship with skeletal muscle fiber types. Results （1） Aerobic exercise promoted both PPAR8 transcription and translation in skeletal muscle suggesting that PPAR8 might play an important role in skeletal muscle adaptation to exercise. （2） Aerobic exercise-induced increase in PPAR8 mRNA and protein expression in skeletal muscle caused no change in muscle fiber type implicating that skeletal muscle fiber type might be affected by both cellular metabolism and some other unknown factors.

关 键 词：有氧耐力运动 肌纤维类型 PPAR8 

分 类 号：G804.7[文化科学—运动人体科学]