人参二醇皂苷对失血性休克-内毒素二次打击大鼠肺组织一氧化氮含量的影响  

The effect of panoxadiol saponin on NO content in lung tissue of the two hits rats with hemorrhagic shock-endotoxin

作　　者：于振香[1] 左孟华[1] 彭丽萍[1] 刘喜春[2] 康劲松[2] 赵雪俭[2] 

机构地区：[1]吉林大学第一医院呼吸内科,吉林长春130021 [2]吉林大学基础医学院病理生理教研室

出　　处：《中国老年学杂志》2008年第1期7-9,共3页Chinese Journal of Gerontology

基　　金：吉林省科委自然科学基金资助(No20030551-3)

摘　　要：目的探讨失血性休克复合内毒素二次打击大鼠肺组织一氧化氮(NO)含量的变化及人参二醇皂苷(PDS)对其影响。方法利用失血性休克对Wistar大鼠制造首次打击,给予地塞米松(Dex)或PDS预治疗,之后腹腔注入脂多糖(LPS)进行第二次打击,二次打击6h后处死大鼠,取肺脏组织进行一氧化氮合酶(NOS)、诱导型NOS(iNOS)活力和NO代谢产物NO2-/NO3-含量的测定。结果二次打击的大鼠肺脏组织NOS、iNOS活力水平及NO2-/NO3-均明显高于假手术对照组(P<0.05),而经过Dex或PDS处理的大鼠肺脏组织NOS、iNOS活力水平及NO2-/NO3-与二次打击大鼠相比均明显降低(P<0.05)。结论失血-内毒素二次打击时,NO产生过多可能参与了急性肺损伤的发生,PDS可通过抑制NO的产生从而达到保护肺脏的作用。Objective To explore the changes of NO contents in the rats with two hits hemorrhagic shock-endotoxin and the effect of panoxadiol saponin （PDS） on it. Methods Wistar rats firstly intervened by hemorrhagic shock were pretreated by dexamethasone （Dex） or PDS, then treated by intra-abdominal injection with lipopolysaccharide （LPS） to be killed 6 h later. NOS, iNOS activities and NO metabolic product NO2^-/NO3^- contents in lung tissue were determined. Results NOS and iNOS activities and NO2^-/NO3^- in lung tissue of two hits rats were significantly higher than those in sham-operated controls（P 〈 0.05 ）. But NOS and iNOS activities and NO2^-/NO3^- in rats treated with Dex or PDS were significantly lower than those in two hits rats （P 〈 0.05）. Conclusions Hemorrhagic shock-endotoxin two hits induced excessive NO product might participate in occurrence of acute lung injury. PDS could protect lung through inhibiting NO product.

关 键 词：急性肺损伤 二次打击 内毒素 人参二醇皂苷 一氧化氮 

分 类 号：R-363[医药卫生] R605.971