红谷霉素生料发酵工艺的研究  

Studies on Unsterile Fermentation Process of Honggumycin

作　　者：熊智强[1] 涂晓嵘[2] 涂国全[2] 

机构地区：[1]华东理工大学生物反应器国家重点实验室国家生化工程技术研究中心,上海200237 [2]江西农业大学生物科学与工程学院,南昌330045

出　　处：《中国生物工程杂志》2008年第1期76-79,共4页China Biotechnology

基　　金：国家教育部重点项目(2003-03072);江西省自然科学基金(0530010),资助项目

摘　　要：红谷霉素是链霉菌702发酵所产的一种生物活性物质,具有较强抗细菌活性。在摇瓶条件下,对链霉菌702生料发酵生产红谷霉素进行研究,采用单因素实验探索生料发酵工艺,筛选出摇瓶生料发酵条件为7.5%的种子接种量和添加抗菌剂1.5ml。在此条件下进行生料发酵,使红谷霉素摇瓶发酵产量达到1.46g/L,比常规灭菌发酵产量提高25.7%,实验结果表明红谷霉素生料发酵是一种潜在可行的发酵方法,不但可以很好地节约发酵成本,还可以提高发酵产量。Honggumycin, which showed a significant antibacterial activity, was a bioactive compound obtained by the fermentation broth of process of honggumycin produced by Streptomyces 702. Under the shaking-flask culture, unsterile fermentation Streptomyces 702 were studied by single-factor experiment design. The optimum unsterile shaking-flask cultivation condition consisted of： 7.5% of inoculation size （ v/v）, 1.5ml of antimicrobial agent addition. Under the optimal conditions, honggumycin yield of unsterile fermentation was up to 1.46g/L, which was increased by 25.7% than that of sterile fermentation. The result showed that fermentation for Streptomyces 702 under non-sterile conditions was an potentially efficient fermentation mode, which not only saved fermentation cost, but also increased fermentation productivity.

关 键 词：链霉菌702 红谷霉素 生料发酵 

分 类 号：Q939[生物学—微生物学]