白细胞介素-10基因多态性与卵巢癌的相关性研究  

Association of interleukin-10 polymorphisms with susceptibility to ovarian cancer in Chinese Han population.

作　　者：何秀萍[1] 王丽丽[1] 赵辉[1] 吴迪[1] 汤海蒂[1] 曹峰林[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院哈尔滨医科大学干细胞与生殖医学研究所,哈尔滨150001

出　　处：《中国优生与遗传杂志》2008年第1期37-38,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨白细胞介素-10(IL-10)基因启动子区域-1082、-819和-592位点单核苷酸多态性与卵巢癌的关系。方法用序列特异性引物聚合酶链(PCR-SSP)技术,检测33例卵巢癌患者和90例正常对照组IL-10基因多态性。结果-819位点卵巢癌患者C/T基因型频率显著高于健康对照组(45.5%比21.0%,P<0.05),C/C和T/T基因型频率虽然低于对照组(分别为6.1%比20.0%和48.4%比59.0%),但是差异无统计学意义(P>0.05);-592位点卵巢癌患者C/A基因型频率显著高于健康对照组(45.5%比21.0%,P<0.05),C/C和A/A基因型频率虽然低于对照组(分别为6.1%比20.0%和48.4%比59.0%),但是差异无统计学意义(P>0.05);-1082位点基因型频率在卵巢癌患者和正常对照组间差异无统计学意义(P>0.05)。-1082、-819、-592位点等位基因频率在卵巢癌患者和正常对照组间差异均无统计学意义。结论IL-10基因启动子区域-819C/T和-592C/A基因型可能与卵巢癌的发生有关。Objective：To investigate whether three diallelic polymorphisms at-1082,-819 and-592 in the promoter region of the IL-10 gene were associated with ovarian carcinoma.Methods：The IL-10 gene-1082,-819 and-592 position polymorphisms were analyzed by sequence specific primers polymerase chain reaction（PCR-SSP） methods in 33 patients with ovary carcinoma and 90 healthy controls.Results：Compared with healthy subjects（HS）,ovarian carcinoma patients had a great frequency of C/T genotype at IL-10 gene promoter-819 position（45.5% vs 21.0%,P〈0.05）,the frequencies of-819 C/C and T/T genotype were not significantly different（6.1% vs 20.0% and 48.4% vs 59.0%,P〉0.05）.ovarian carcinoma patients also had a great frequency of-592 C/A genotype at IL-10 compared with HS（45.5% vs 21.0%,P〈0.05）,the frequencies of-529 C/C and A/A genotype were not significantly different（6.1% vs 20.0% and 48.4% vs 59.0%,P〉0.05）.No significant difference was found in genotype at IL-10 gene promoter-1082 position.No significant difference was found in allele frequency at IL-10 gene-1082,-819 and-529 positions.Conclusion：The presence of-819C/T and C/A genotype may be related to development of ovarian carcinoma.

关 键 词：卵巢癌 白细胞介素-10 基因多态性 

分 类 号：R737.31[医药卫生—肿瘤]