一种实验性脊髓空洞前状态动物模型的建立  

Establishment of experimental model of Presyrinx state in rabbits

作　　者：孙国柱[1] 赵宗茂[1] 张更申[1] 张庆俊[2] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000 [2]北京大学人民医院神经外科

出　　处：《脑与神经疾病杂志》2008年第1期8-10,共3页Journal of Brain and Nervous Diseases

基　　金：河北省科技厅资助项目(0620611136D-11);河北省卫生厅资助项目(06085)

摘　　要：目的:建立一种新西兰兔脊髓空洞前状态动物模型。方法:新西兰兔64只,随机分为Kaolin组、生理盐水组和假手术组,其中Kaolin组48只,经皮枕大池穿刺注入25%Kaolin混悬液0.6ml,分别于Kaolin注射后第1d、3d、7d、14d、21d、28d后常规行MRI观察后处死,取出上颈髓行组织学观察。结果:Kaolin组动物自Kaolin注入后3d出现缺血水肿(72.78±0.88%),7～14d达高峰(72.94±0.64%),21d减轻(70.21±0.49%);在T1WI表现为略低信号,T2WI为高信号且不能为FLAIR序列所抑制。至28d动物出现上颈髓空洞。结论:经皮枕大池Kaolin注射法可以制作出一种稳定性好、可重复率高的脊髓空洞前状态动物模型。Objective： To establish a sort of adult model of presyrinx state in Newland rabbits. Methods： 64 rabbits were randomly divided into Kaolin group, normal saline group and sham-operation group. In former group, 25% kaolin solution were percutaneously punctured and injected into the cisterna magna of animals. MRI were performed on all animals at 1d,3d,7d, 14d,21d after operation respectively before animals were sacrificed, then histological observes were car ried out on the upper cervical cord. Results： Water content assay and histological observation showed that spinal cord edema in Kaolin group animals appeared at the 1st day（68.46±0.70% ）after kaolin injection, was more prominent at 3rd day （72.78±0.88%）, reached its peak at 7th day （72.94±0.64%）, and declined slowly after 3 weeks（70.21±0.49%）; MRI found the slightly lower signal in T1WI, high signal in T2WI in upper cervical cord which could be suppressed in FLAIR. Until 28d,syrinx occurred in upper cervical cord. Conclusion： percutaneous puncture of Kaolin solution into cisterna magna is an idea animal model wih better stabilization and high reproducibility for studying presyrinx state.

关 键 词：空洞前状态 新西兰兔模型 KAOLIN 

分 类 号：R744.4[医药卫生—神经病学与精神病学]