龈沟液微量样本中多种成分的检测  

Detection of various component in the trace sample of gingival crevicular fluid

作　　者：陈智滨[1] 孙晓军[1] 寇传哲[2] 孟焕新[1] 

机构地区：[1]北京大学口腔医学院·口腔医院牙周科,北京100081 [2]北京朝阳医院京西院区口腔科

出　　处：《北京大学学报（医学版）》2008年第1期57-59,共3页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30271411,30471882)资助~~

摘　　要：目的:以龈沟液(gingival crevicular fluid,GCF)样本为例,探寻微量样本中多种成分的检测方法。方法:根据酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)的基本原理,确认待检测成分之间无交叉反应,样本与包被抗体在4℃下进行低温反应,重复使用同一样本。39份龈沟液来自4例轻、中度牙周炎患者,龈沟液洗提液分为两份,一份样本纳入A组,用ELISA方法先检测白细胞介素6(interleukin-6,IL-6),再用此样本检测白细胞介素1β(interleukin-1β,IL-1β);另一份样本纳入B组,直接进行IL-1β检测。比较A组与B组IL-1β的检测结果。65份龈沟液样本选自9例来北京大学口腔医院修复科就诊患者,龈沟液洗提液分为两份,一份样本纳入C组,用ELISA方法先检测肿瘤坏死因子(tumor necrosis factor-α,TNF-α)后,再用此样本检测弹性蛋白酶;另一份纳入B组,直接进行弹性蛋白酶检测。比较A组与B组弹性蛋白酶的检测结果。结果:A和B组IL-1β光密度值分别为(0.5±0.4)和(0.5±0.4),差异无统计学意义(P=0.136),且高度相关(r=0.993,P=0.000);C和D组弹性蛋白酶吸光度值分别为(1.1±0.6)和(1.1±0.6),差异无统计学意义(P=0.943),且高度相关(r=0.979,P=0.000)。结论:通过重复使用同一样本,可以达到用微量样本检测多种成分的目的。Objective： To evaluate methods of detecting various component in the trace sample of the gingival crevicular fluid （GCF）. Methods： One sample of GCF was collected from four patients with mild or moderate periodontitis. At first, one half （group A）of every sample was measured for interleukin- 6 （IL-6） by enzyme-linked immunosorbent assay （ELISA） and then detected for Interleukin-1β （IL-1β ） in the same-sample . Another half （group B） of the sample was used as control for the measurement of Interleukin-1β （ IL-1β）. Another sample of GCF was collected from nine patients with abutment and nonabutment because of the distal extension teeth lost resulted from the chronic periodontitis. At first, one half （group C） of the sample was detected for tumor necrosis factor -α （TNF-α） by ELISA and then measured for elastase in the same sample. Another half （ group D） of the sample was used as control for the measurement of elastase. Results： There was no significant difference of both the measured value of IL-1β absorbency （group A and B are 0.5 ± 0.4 and 0.5 ± 0.4, respectively, P = 0. 136） and that of elastase absorbency （ group C and D are 1.1 ± 0.6 and 1.1 ± 0.6, respectively, P = 0.943 ） between the original elution and the sample after detection of IL-6 or TNF-α in the test 1 and test 2, respectively. Moreover, the values showed high correlation （ r = 0. 993, P = 0. 000 in the group A and B ; r = 0. 979, P = 0. 000 in the group C and D）. Conclusion： It is practicable that one sample of GCF can be reused to examine for several components of GCF.

关 键 词：牙龈缝液 白细胞介素1 胰弹性蛋白酶 

分 类 号：R781.42[医药卫生—口腔医学]