应用光化学法建立兔眼视网膜静脉分支阻塞模型  

Establishment of retinal vein occlusion with photochemical method

作　　者：李立婕[1] 金中秋[1] 

机构地区：[1]广州军区武汉总医院眼科,湖北省武汉市430070

出　　处：《眼科新进展》2008年第2期90-93,共4页Recent Advances in Ophthalmology

摘　　要：目的采用光化学法建立兔眼视网膜静脉分支阻塞模型。方法30只健康青紫蓝兔随机分为3组,每组10只,A组激光光凝双侧静脉,B组激光光凝单侧动脉及双侧静脉,C组为空白对照。经耳缘静脉注入孟加拉玫红(50mg·kg-1),随后按照分组氪-绿激光光凝封闭视网膜血管,行眼底照相及荧光造影证实视网膜静脉阻塞模型的建立,形成后于1d、5d、7d、21d、30d行眼底检查及荧光造影检查,确定模型建立后眼底的变化情况。于光凝后1d、30d处死动物,摘除眼球行光镜和电镜观察。结果眼底荧光造影结果显示,A组6眼阻塞血管再通,眼底仅表现部分静脉远端迂曲,未见明显视网膜静脉阻塞眼底改变,B组9眼均有明显视网膜静脉阻塞表现,C组眼底无改变。光凝后1d,光镜、电镜下视网膜均未见明显异常。30d光镜、电镜观察见B组视网膜静脉血栓形成,并出现视网膜分支静脉阻塞相应病理改变。结论氪-绿激光光凝兔眼视网膜部分动脉及双侧静脉可以产生视网膜静脉分支阻塞的典型表现,该法可为临床研究视网膜静脉阻塞提供较为安全、可靠、稳定的动物模型。Objective To establish reliable,stable and reproducible animal models of branch retinal vein occlusion （BRVO） with photochemical method in rabbits＇ eyes. Methods Thirty healthy experimental rabbits were divided into 3 groups randomly, 10 eyes in each group. Group A： Krypton-green laser photocoagulated bilateral retinal veins;Group B： Krypton-green laser photocoagulated bilateral retinal veins and unilateral artery;Group C：Blank. Rose bengal（ 50 mg· kg^-1 ） solution were injected via ear vein, and retinal vessels were blocked with krypton-green laser photocoagulates. Funduscope observation and fimdus fluorescein angiography were used to confirm the establishment of animal models and changes offimdus at 1 day,5 days,7 days,21 days and 30 days after the establishment of animal models. Rabbit were killed at 1 day and 30 days after laser photocoagulates, and eyeballs were examined with lightscope and electroscope. Results In group A,the retinal vein recanalized in 6 rabbits,with circuity of the distal end of fundus vein and no visible retinal vein occlusion;In group B,there was obvious BRVO,and no changes in group C. Structure of retina was generally normal under light and electron microscope at the 1st day after photocoagulation. The formation of retina vein thromb was seen and retinal showed histopathologic changes corresponding BRVO at 30 days after photocoagulation. Conclusion Embolizing bilateral veins and monolateral artery with kryptongreen laser induces the typical BRVO symptoms, which provide a reliable, stable, and reproducible RVO experimental animal model. [ Rec Adv Ophthalmol 2008 ; 28 （ 2 ） ： 90.93]

关 键 词：光化学 视网膜分支静脉阻塞 动物模型 

分 类 号：R774.1[医药卫生—眼科]