川芎嗪对环丙沙星在角膜穿孔伤兔眼眼内渗透性的影响  

Ligustrazine on ocular endosmosis of ciprofloxacin in rabbit eyes of corneal perforating injury

作　　者：周蓓 林晓峰[2] 吴伟[2] 叶茂果 胡冬梅 李青[2] 

机构地区：[1]广东省广州市天河区中心医院眼科,510655 [2]中山大学中山眼科中心,广东省广州市510060

出　　处：《眼科新进展》2008年第2期100-102,共3页Recent Advances in Ophthalmology

基　　金：广东省中医药管理局经费资助(编号:1050065)~~

摘　　要：目的研究兔角膜穿孔伤后血-眼屏障的改变及川芎嗪对环丙沙星在兔眼角膜穿孔伤眼内渗透性的影响。方法18只新西兰兔,随机分为A、B、C3组。A、B组各眼行角膜穿孔伤,1h后A组静脉注射环丙沙星,B组静脉注射川芎嗪和环丙沙星;C组为对照组单纯静脉注射环丙沙星。用药后0.5h,取房水及玻璃体,双缩脲法测定房水蛋白含量,反向高效液相色谱法测定房水及玻璃体环丙沙星浓度。结果A、B和C组房水蛋白含量分别为(11.977±5.774)g·L-1、(14.767±5.814)g·L-1和(2.741±1.193)g·L-1,A、B组显著高于C组(P<0.01);A、B和C组房水环丙沙星浓度分别是(1.390±0.284)mg·L-1、(1.390±0.284)mg·L-1和(0.655±0.291)mg·L-1,A、B组显著高于C组(P<0.01);A、B2组房水蛋白的含量与环丙沙星的浓度呈正相关(P<0.01),但A、B2组房水蛋白浓度及环丙沙星浓度比较差异无统计学意义(P>0.05);3组玻璃体中环丙沙星浓度均很低,分别是(0.073±0.059)mg·L-1、(0.085±0.041)mg·L-1和(0.064±0.051)mg·L-1,3组比较差异无统计学意义(P>0.05)。结论角膜穿孔伤增加血-房水屏障通透性,使房水中环丙沙星水平明显提高,浓度显著高于大部分眼内致病菌90%菌株最小抑菌浓度。川芎嗪不影响外伤眼血-房水屏障,不能增加环丙沙星在角膜穿孔伤兔眼眼内渗透性,可作为角膜穿孔伤后改善血循环用药。Objective To investigate the changes of blood-ocular barrier and effect of ligustrazine on the ocular endosmosis of intravenous ciprofloxacin in rabbit eyes of corneal perforating injury. Methods Eighteen New Zealand rabbits were randomly divided into 3 groups. Group A and B received surgically corneal perforating injury to each eye. After 1 hour, each rabbit received intravenous infusion of ciprofloxacin in group A and intravenous infusion of ciprofloxacin following ligustrazine in group B. Group C served as control group with only intravenous infusion of ciprofloxacin. Ciprofloxacin concentration of aqueous humor and vitreous body was measured 0.5 hour after the intravenous injection by reversed phase high performance liquid chromatograph （RP-HPCL） and the protein concentration of aqueous humor was measured with biuret conorimetric method simultaneously in all eyes. Results The protein concentrations of aqueous humor in experimental groups （ group A and B）were, （ 11. 977 ± 5.774 ）g · L^-1 and（ 14.767 ±5.814 ）g · L^-1 ,significantly higher than that of control group, （2.741 ± 1. 193 ） g· L^-1 （ P 〈 0.01 ）. The ciprofloxacin concentration of aqueous humor in experimental groups （group A and B） were,（ 1. 390 ±0.284）mg · L^-1 and （ 1. 390 ± 0. 284 ） mg · L^-1, significantly higher that of control group, （ 0. 655 ± 0. 291 ） mg · L^-1. There was positive correlation between protein concentration and ciprofloxacin concentration in group A and B （ P 〈 0. 01 ）, but no significant difference （P〉0.05）. The eiprofloxacin concentrations were（0.073 ± 0.059 ）mg · L^-1 , （0.085 ±0.041 ）mg · L^-1 and （0.064 ±0.051 ）mg · L^-1 in group A,B and C,respectively. Conclusion The permeability of blood-retina barrier is increased after Perforating corneal trauma, which results in increased ciprofloxacin concentration in aqueous humor. Ciprofloxacin levels of aqueous humor after intravenous injection are above the minimum inhibitory concentration （MIC90

关 键 词：角膜穿孔伤 血-眼屏障 环丙沙星 川芎嗪 渗透性 

分 类 号：R772[医药卫生—眼科]