前列腺组织STEAP表达与H2O2水平的关系  

Relationship between STEAP expression and hydrogen peroxide in human prostate tissues

作　　者：潘玉琢[1] 郭丽荣[1] 梁爽[1] 李扬[1] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林大学前列腺疾病防治研究中心,吉林长春130021

出　　处：《中国病理生理杂志》2008年第2期354-356,共3页Chinese Journal of Pathophysiology

基　　金：国家科技部国际重点科技合作基金资助项目(No.2004DFB02000)

摘　　要：目的:探讨STEAP基因的功能。方法:应用RT-PCR、免疫印迹的方法,检测STEAP mRNA和蛋白在国人正常前列腺、前列腺癌组织中的表达水平。应用钼酸还原法检测正常前列腺内腺、外腺及前列腺癌组织H2O2水平,同时应用黄嘌呤氧化法测定3种组织中SOD水平,分析其与STEAP表达的相关性。结果:国人前列腺癌STEAP mRNA和蛋白高表达;前列腺癌组织中H2O2水平及总SOD水平明显高于正常前列腺。结论:STEAP的功能之一可能是诱导内源性H2O2水平增高,促进细胞快速生长。AIM： To observe the STEAP expression and its function in human prostate tissues. METHODS： The expressions of STEAP mRNA and protein were detected by RT - PCR and Westem blotting. H2O2 and SOD levels were detected by molybdic acid reduction and xanthine oxidation methods respectively. RESULTS： STEAP mRNA and protein were highly expressed in prostate cancer tissues. H2O2 and SOD levels in prostate cancer were obviously higher than those in normal prostate tissue. CONCLUSION： The function of STEAP gene is possibly to induce intracellular H2O2 and promote cell growth.

关 键 词：前列腺肿瘤 过氧化氢 前列腺6个跨膜上皮抗原 

分 类 号：R363[医药卫生—病理学]