E-cadherin、CD44v6和PCNA在非小细胞肺癌组织中的表达及意义  

Expression and Significance of E-cadherin, CD44v6, and Proliferating Cell Nuclear Antigen in Non-small Cell Lung Cancer

作　　者：翁密霞[1] 吴翠环[2] 杨秀萍[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院病理科,湖北武汉430022 [2]华中科技大学同济医学院基础医学院病理学系,湖北武汉430030

出　　处：《癌症》2008年第2期191-195,共5页Chinese Journal of Cancer

摘　　要：背景与目的:上皮钙依赖粘附蛋白(E-cadherin,E-cad)、CD44v6和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在恶性肿瘤侵袭与转移中发挥重要作用。本研究旨在探讨E-cad、CD44v6和PCNA在非小细胞肺癌(non-small cell lung cancer,NSCLC)及癌旁组织中的表达与NSCLC的侵袭、转移及预后的关系。方法:应用免疫组化EnVision法检测86例NSCLC组织及40例癌旁组织中E-cad、CD44v6和PCNA的表达,并分析与NSCLC的侵袭、转移及预后的关系。结果:E-cad在肺癌组织中的表达率为53.5%(46/86),显著低于癌旁组织(80.0%)(P<0.05),且与NSCLC的分化程度、淋巴结转移和TNM分期有关(P<0.05);CD44v6在肺癌组织中的表达率为44.2%(38/86),在癌旁组织中无表达,且在鳞癌中的表达率(54.0%)显著高于腺癌(30.6%)(P<0.05),并且与淋巴结转移和TNM分期有关(P<0.05);PCNA在肺癌组织中的表达率为48.8%(42/86),在癌旁组织中无表达,且在淋巴结转移组与未转移组间的表达差异有统计学意义(P<0.05)。E-cad与PCNA的表达呈显著性负相关(r=-0.554,P<0.05),而CD44v6与PCNA的表达呈显著性正相关(r=0.688,P<0.05)。单因素生存分析显示E-cad、CD44v6及PCNA的表达与NSCLC患者预后有关。Cox比例风险模型进行多因素生存分析显示;E-cad与临床分期是有意义的预后指标(P<0.05)。结论:E-cad、CD44v6及PCNA的表达与NSCLC的侵袭和转移相关。在NSCLC中联合检测三者的表达对判断预后有参考价值。BACKGROUND ＆ OBJECTIVE： E-cadherin （E-cad）, CD44v6 and proliferating cell nuclear antigen （PCNA） play important roles in invasion and metastasis of cancers. This study was to investigate the correlations of the expression of E-cad, CD44v6, and PCNA to the invasion, metastasis, and prognosis of non-small cell lung cancer （NSCLC）. METHODS： The expression of E-cad, CD44v6 and PCNA in 86 specimens of NSCLC and 40 specimens of adjacent normal tissues were detected by EnVision immunohistochemistry. RESULTS： The high expression rate of E-cad was significantly lower in NSCLC than in adjacent normal tissues （53.5% vs. 80.0%, P〈0.05）. E-cad staining in NSCLC tissues was correlated to differentiation, lymph node metastasis and TNM stage （P〈0.05）. The high expression rate of CD44v6 was 44.2% in NSCLC, and 0 in adjacent normal tissues. CD44v6 staining in NSCLC tissues was correlated to classification, lymph node metastasis and TNM stage （P〈 0.05）. The high expression rate of PCNA was 48.8% in NSCLC, and 0 in adjacent normal tissues. PCNA staining was correlated to lymph node metastasis （P〈0.05）. PCNA expression was negatively correlated to E-cad expression （r=-0.554, P〈0.05）, and positively correlated to CD44v6 expression （r=0.688, P〈0.05）. Univariate analysis indicated that E-cad, CD44v6, and PCNA were prognostic factors of NSCLC. Multivariate analysis showed that E-cad and TNM stage were independent prognostic indicators （P〈0.05）. CONCLUSIONS： E-cad, CD44v6 and PCNA play important roles in invasion and metastasis of NSCLC. The expression of E-cad, CD44v6 and PCNA may be of prognostic value in patients with NSCLC.

关 键 词：肺肿瘤 癌 非小细胞 E-CADHERIN CD44 PCNA 预后 

分 类 号：R734.2[医药卫生—肿瘤]