多巴胺激动药对大鼠垂体组织表达Pit-1作用的研究  

Effect of dopamine agonist on expression of pit-1 in rat pituitary tissue

作　　者：刘亚力[1] 邱文娟[1] 杨雪梅[1] 徐春[1] 

机构地区：[1]武警总医院内分泌科,北京100039

出　　处：《武警医学》2008年第1期11-13,共3页Medical Journal of the Chinese People's Armed Police Force

基　　金：首都医学发展基金(中医药类)(2001-Ⅲ-21);武警总医院科研基金(WKH2005070)

摘　　要：目的研究多巴胺激动药对大鼠垂体组织表达Pit-1的作用。方法成年雄性Wistar大鼠,随机分为3组,催乳素瘤(PRL)组、对照组和诺果宁组,各组按照实验程序操作。8周后处死动物,测定大鼠血清PRL水平;用免疫组化方法显示垂体组织PRL蛋白的表达和分布;用RT-PCR方法检测Pit-1mRNA在两组垂体组织中的表达,以β-actin作为内参照,借助于计算机凝胶成像系统分析表达量。结果诺果宁组大鼠血清PRL水平和垂体重量均明显低于PRL瘤组(P<0.01),Pit-1mRNA水平在诺果宁组较PRL瘤组明显降低(P<0.01)。结论降低Pit-1的表达水平可能是诺果宁抗PRL瘤的重要机制之一。Objective To study the effect of dopamine agonist on the expression of Pit - 1 in pituitary tissue. Methods Prolactinoma rat model was developed. Male adult Wistar rats were divided into 3 groups at random. Each rat in control group was subseutaneously implanted with a blank implant. Rats in prolactinoma group were implanted with diethyl-stilbestrol （DES） - containing implants. Rats in Norprolac group were also implanted with DES- containing implants, meanwhile Norprolac were orally administrated[ 30 μg/（kg ·day） ] to each rats in Norprolac （ quinagolide ） group. After 8 weeks, all the animals were sacrificed. Each pituitary gland was weighed. Serum prolactin（PRL） levels were measured by RIA.Pit- 1 mRNA levels in pituitary tissue were measured by RT-PCR, Results The weight of pituitary gland and PRL level in Norprolac group were significantly lower than those in prolactinoma group（ P 〈 0.001 ）. The expression level of Pit - 1 mRNA in Norprolac group was significantly lower than that in prolactinoma group （ P 〈 0.001 ）. Conclusions The decrease of Pit- 1 mRNA level may be involved in the mechanism of anti- prolactinoma effect of Norprolac.

关 键 词：雌激素 催乳素瘤 大鼠 Pit-1 多巴胺激动药 

分 类 号：R736.4[医药卫生—肿瘤]