淫羊藿黄酮的体外抗氧化作用  

In vitro Antioxidant Properties of Epmedium flavonoids

作　　者：杨建雄[1] 杨丽霞[1] 杨晨[2] 包宇[1] 黄亚亚[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]西安交通大学医学院医学生物学与遗传学教研室,西安710061

出　　处：《天然产物研究与开发》2007年第B05期72-75,共4页Natural Product Research and Development

基　　金：陕西省科技攻关项目（2004K18-G10）

摘　　要：为研究淫羊藿黄酮（Epmedium；lavonoids，EF）的体外抗氧化作用，用水杨酸法研究FLF清除·OH的效果，并测定了EF对连苯三酚自氧化体系的抑制作用。用硫代巴比妥酸（TBA）法测定了小鼠4种器官及肝线粒体、微粒体中的丙二醛（MDA）含量。用分光光度法测定了小鼠红细胞溶血和肝线粒体膨胀程度。结果表明，EF可以清除·OH，抑制连苯三酚自氧化，并抑制·OH所致丙二醛的产生，减少红细胞溶血，减轻肝线粒体膨胀程度。说明EF具有明显的抗氧化活性。To study the in vitro antioxidant activity of the Epmedium flavonoids （ EF）, the scavenging effect of EF on hydroxyl radicals（ · OH ） was studied by salicylic acid method, and serf-oxidation inhibition was studied using the pyrogallol. The contents of MDA in 4 organs, and in mitochondria and microsome of liver were measured by TBA assay. The extent of erythrocatalysis and the swelling extent of mitochondria of liver were detected by spectrophotometric methods. The result showed that EF had the · OH and O2 · scavenging activity. It could inhibit the generation of MDA, the swelling of mitochondria, and the hemolysis of red blood cells. The conclusion was that EF have the antioxidant activity.

关 键 词：淫羊藿黄酮 抗氧化 体外 自由基 

分 类 号：R151.2[医药卫生—营养与食品卫生学]