不同参数电刺激上矢状窦对大鼠硬脑膜血流量的影响  

Effect of Different Electric Stimulation Parameters on Superior Sagittal Sinus Dura Blood Flow in Rats

作　　者：董钊[1] 于生元[1] 赵秀梅[2] 姜磊[1] 李凤鹏[1] 邱恩超[1] 韩岳[2] 刘秀华[2] 

机构地区：[1]中国人民解放军总医院神经内科,北京100853 [2]中国人民解放军总医院病理生理学研究室,北京100853

出　　处：《微循环学杂志》2008年第1期13-14,19,共3页Chinese Journal of Microcirculation

基　　金：国家自然科学基金项目(30570264)

摘　　要：目的:比较不同参数电刺激上矢状窦对大鼠硬脑膜血流量的影响。方法:使用L9(34)正交表,采用正交实验设计确立电刺激参数为频率、脉宽、电压,每个因素各分为3个实验水平,分别是1Hz、5Hz、30Hz,250μs、500μs、1000μs和3V、6V、9V。暴露SD大鼠上矢状窦旁硬脑膜,按照正交表的刺激参数组合顺序刺激硬脑膜建立偏头痛模型,激光多普勒血流量图像仪测定硬脑膜血流量,比较不同实验组血流量较基线的增加比率。结果:3种水平刺激参数对硬脑膜血流量的影响由大到小依次为频率、脉宽、电压,只有频率对硬膜血流量的影响有显著性统计学意义(F=27.728,P<0.05),而脉宽(F=2.855,P>0.05)和电压(F=2.418,P>0.05)的影响无统计学意义。结论:刺激频率是电刺激大鼠上矢状窦偏头痛模型的3种参数中影响硬膜血流量的主要因素。Objective:To analyze the effect of different electric stimulation parameters on superior sagittal sinus dura matter blood flow in rats.Method:By using orthogonal layout of L9(34)three electric stimulation parameters which were divided into three levels were evaluated:frequency(1 Hz,5 Hz,30 Hz),duration(250 μs,500 μs,1 000 μs)and voltage(3 V,6 V,9 V).Dual matter adjacent to the superior sagittal sinus in male rats was stimulated applying different combinations of stimulation parameters and laser doppler perfusion imager(LDI)was used to measure dual matter blood flow in different groups.Results:The effect of frequency on dura matter blood flow was significant(F =27.728,P =0.035)but significant difference in other stimulation parameters was not found(duration P=0.259 and voltage P=0.293).Conclusion:Frequency is the most important factor among three electric stimulation parameters when applying transformed superior sinus stimulation migraine model.

关 键 词：电刺激 脑膜血流量 上矢状窦 神经源性炎症 血管源学说 大鼠 炎症反应学说 病理生理过程 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R971[医药卫生—临床医学]